Membrane Trafficking Methods and Protocols
This detailed volume presents a series of methods exploring membrane trafficking research, ranging from genetics and high-resolution imaging to in vitro biochemical and biophysical assays. Covering virtually all the major trafficking branches, the book delves into the exocytic pathway, which focuses...
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| Format: | eBook |
| Language: | English |
| Published: |
New York, NY
Humana
2022, 2022
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| Edition: | 1st ed. 2022 |
| Series: | Methods in Molecular Biology
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| Subjects: | |
| Online Access: | |
| Collection: | Springer eBooks 2005- - Collection details see MPG.ReNa |
Table of Contents:
- Characterizing Membrane Traffic in the Early Secretory Pathway Using the RUSH Retention System
- Monitoring Intra-Golgi Transport with Acute Spatiotemporal Control of a Synthetic Cargo
- Imaging Secretory Granule Budding from the Trans-Golgi Network Using Retention Using Selective Hook (RUSH)
- Spatial and Temporal Control of Protein Secretion with Light
- Generation of Endogenously Tagged Membrane Trafficking Regulators Using CRISPR Genome Editing
- Determine the Function of the Exocyst in Vesicle Tethering by Ectopic Targeting
- Imaging Insulin Granule Dynamics in Human Pancreatic β-Cells Using Total Internal Reflection Fluorescence (TIRF) Microscopy
- Monitoring Synaptic Exocytosis Using a Whole-Cell Patch Clamp Technique
- Live-Cell Super-Resolution Imaging of Retrograde Axonal Trafficking Using Pulse-Chase Labeling in Cultured Hippocampal Neurons
- Electron Tomographic Methods for Studying Organelles of the Murine Chemical Synapse
- In Vitro Reconstitution Studies of SNAREs and Their Regulators Mediating GLUT4 Vesicle Fusion
- Imaging Single Vesicle Exocytosis with Total Internal Reflection Fluorescence Microscopy (TIRFM)
- Localizing Proteins on Single Trafficking Organelles in 3D with Semisynthetic Gold Labeling and Platinum Replica Electron Microscopy
- Endocytosis Assays Using Cleavable Fluorescent Dyes
- Reconstituting and Purifying Assembly Intermediates of Clathrin Adaptors AP1 and AP2
- Monitoring Endosomal Cargo Retrieval to the Trans-Golgi Network by Microscopic and Biochemical Approaches
- Measuring Plasma Membrane Recycling Using Microscopic and Biochemical Approaches
- Light Scattering Techniques to Assess Self-Assembly and Hydrodynamics of Membrane Trafficking Proteins
- Measurement of Lysosome Positioning by Shell Analysis and Line Scan
- Optogenetic Control of Membrane Trafficking Using Light-Activated Reversible Inhibition by Assembly Trap of Intracellular Membranes (IM-LARIAT)
- Generating Custom Pooled CRISPR Libraries for Genetic Dissectionof Biological Pathways
- Studying Unconventional Secretion of Misfolded Proteins in Cultured Cells and Primary Neurons
- Single-Molecule Optical Tweezers Study of Protein-Membrane Interactions
- Generation of Hybrid Extracellular Vesicles by Fusion with Functionalized Liposomes
- Quantitative Measurement of Extracellular Vesicle Content Delivery within Acceptor Cells