Effector-Triggered Immunity Methods and Protocols
This volume presents a collection of protocols to study effector-triggered immunity (ETI) in both plants and animals from eminent groups in the field. The chapters in this book cover topics such as genetic manipulation of plant and animal pathogens, host cells, and the analysis of key host responses...
| Other Authors: | , |
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| Format: | eBook |
| Language: | English |
| Published: |
New York, NY
Springer US
2022, 2022
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| Edition: | 1st ed. 2022 |
| Series: | Methods in Molecular Biology
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| Subjects: | |
| Online Access: | |
| Collection: | Springer eBooks 2005- - Collection details see MPG.ReNa |
Table of Contents:
- A Brief Introduction to Effector-Triggered Immunity
- Lambda Red Recombineering in Shigella flexneri
- Using Genomic Deletion Mutants to Investigate Effector-Triggered Immunity during Legionella pneumophila Infection
- Methods of Bacterial Membrane Vesicle Production, Purification, Quantification, and Examination of their Immunogenic Functions
- Gene Gun-Mediated Transient Gene Expression for Functional Studies in Plant Immunity
- Host Induced Gene Silencing using BPMV on Soybean to Study Genes in the Soybean Rust Fungus Phakopsora pachyrhizi
- Virus-Mediated Protein Overexpression (VOX) in Monocots to Identify and Functionally Characterize Fungal Effectors
- Time-Resolved Fluorescence Microscopy Screens on Host Protein Subversion during Bacterial Cell Invasion
- Assaying RIPK2 Activation by Complex Formation
- Identification of Novel Endogenous NOD Ligands: Quantitative Analysis of Binding Affinities of NOD1 or NOD2 with Sphingosine-1-Phosphate using Microscale Thermophoresis
- A Surface Plasmon Resonance-Based Strategy to Characterize Interactions of NLR Proteins with Associated Factors
- Functional Assessment of Disease-Associated Pyrin Variants
- An Assay for the Seeding of Homotypic Pyrin Domain Filament Transitions
- Detection of Gasdermin Activation and Lytic Cell Death during Pyroptosis and Apoptosis
- Visualizing Effector Triggered Immunity in Response to Pore-Forming Toxins by Live-Cell Imaging
- Non-Apoptotic Cell Death Control of Neutrophil Extracellular Trap Formation
- Analysis of Bacteria-Triggered Inflammasome-Activation in Neutrophils by Immunoblot
- Quantifying Regulated Mitochondrial Fission in Macrophages
- Live Cell Imaging of T Cell Pyroptosis