Asthma Methods and Protocols

This volume details a collection of laboratory protocols to study asthma in mice and humans. Chapters cover animal models of asthma, methods to measure asthma-related molecules, protocols to detect, isolate, culture and stimulate cells that contribute to asthma in mice and humans, methods to deplete...

Full description

Bibliographic Details
Other Authors: Gorska, Magdalena M. (Editor)
Format: eBook
Language:English
Published: New York, NY Humana 2022, 2022
Edition:1st ed. 2022
Series:Methods in Molecular Biology
Subjects:
Online Access:
Collection: Springer eBooks 2005- - Collection details see MPG.ReNa
Table of Contents:
  • Modelling Asthma in Mice Using Common Aeroallergens
  • Mouse Models of Respiratory Syncytial Virus Infection
  • Modeling Asthma in Mice Using Rhinovirus Infection
  • Mouse Model of Sendai Virus-induced Lung Disease
  • Ozone-Induced Models of Airway Hyperreactivity and Epithelial Injury
  • A Novel Non-Human Primate Model of Nonatopic Asthma
  • Measurement of Airway Hyperresponsiveness in Mice
  • Human Precision-Cut Lung Slices: Generation of and Measurement of Contractility and Relaxation of Small Airways
  • Infection of Human Precision-Cut Lung Slices with the Influenza Virus
  • Human Bronchial Epithelial Cell Culture Models for Cigarette Smoke and Vaping Studies
  • Isolation, Ex Vivo Culture and Stimulation of Tracheal and Nasal Chemosensory Cells
  • Detection, Isolation and Functional Studies of Mouse Pulmonary Group 2 Innate Lymphoid Cells
  • Isolation and Characterization of Conventional and Non-Conventional Type-2 Innate Lymphoid Cells (ILC2s) from Human Peripheral Blood Mononuclear Cells (PBMCs)
  • Detection of Eosinophils in Tissue Sections by Immunohistochemistry
  • Targeting Eosinophils in Mouse Models of Asthma
  • Detection and Isolation of Airway Mast Cell Subsets in Mouse and Human
  • Isolation of Conventional Murine Lung Dendritic Cell Subsets
  • Isolation and Analysis of Macrophage Subsets from the Mouse and Human Lung
  • Mass Cytometry Analysis of Whole Blood Response to an Allergen
  • Methods to Detect Neutrophil Extracellular Traps in Asthma
  • Analysis of Airway Vagal Neurons
  • Protocols to Measure Oxidative Stress and DNA Damage in Asthma