Catharanthus roseus Methods and Protocols
This volume provides updated technical approaches that have been developed to characterize monoterpene indole alkaloid metabolism in C. roseus from metabolite/gene product localization, alkaloid chemical synthesis, candidate gene prediction, transcription factor characterization up to functional gen...
| Other Authors: | , |
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| Format: | eBook |
| Language: | English |
| Published: |
New York, NY
Humana
2022, 2022
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| Edition: | 1st ed. 2022 |
| Series: | Methods in Molecular Biology
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| Subjects: | |
| Online Access: | |
| Collection: | Springer eBooks 2005- - Collection details see MPG.ReNa |
Table of Contents:
- A rapid and efficient vacuum-based Agroinfiltration protocol for transient gene overexpression in leaves of Catharanthus roseus
- Transient gene expression in Catharanthus roseus flower petals using agroinfiltration
- De novo shoot bud induction from the Catharanthus roseus leaf explants and Agrobacterium tumefaciens mediated technique to raise transgenic plants
- Agrobacterium-mediated in planta transformation in Periwinkle
- Predicting Monoterpene Indole Alkaloid related genes from expression data with artificial neural networks
- Discovery and characterization of oxidative enzymes in monoterpenoid indole alkaloid biosynthesis
- Ancestral sequence reconstruction for exploring alkaloid evolution
- Generating an EMS mutant population and rapid mutant screening by thin layer chromatography enables the studies of monoterpenoid indole alkaloids biosynthesis in Catharanthus roseus
- TARGETing transcriptional regulation in the medicinal plant Catharanthus roseus
- Identification and Characterization of Transcription Factors Regulating Terpenoid Indole Alkaloid Biosynthesis in Catharanthus roseus
- From Methylome to Integrative Analysis of Tissue-Specificity
- Tagging and capture of prenylated CaaX-proteins from plant cell cultures
- EASI transformation protocol: An Agrobacterium-mediated transient transformation protocol for Catharanthus roseus seedlings
- RNA in situ hybridization on paraffin-sections to characterize the multicellular compartmentation of plant secondary metabolisms
- Imaging MS analysis in Catharanthus roseus
- Surface-Assisted Laser Desorption/Ionization Imaging Mass Spectrometry (SALDI-IMS)-Based Detection of Vinca Alkaloids Distribution in the Petal of Madagascar Periwinkle
- Sample preparation, data acquisition, and data analysis for 15N-labeled and non-labeled monoterpene indole alkaloids in Catharanthus roseus
- Studying iridoid transport in Catharanthus roseus by grafting
- Pictet-Spengler reaction for the chemical synthesis of strictosidine
- Implementation of a MS/MS spectral library for Monoterpene Indole Alkaloids
- Semi-Synthesis of Bis-Indole Alkaloid (–)-Melodinine K Enabled by a Combination of Biotransformation and Chemical Synthesis
- RNA-seq analysis towards monoterpene indole alkaloid biosynthetic pathway elucidation in Catharanthus roseus