SARS-CoV-2 Methods and Protocols

This detailed volume provides the increasing number of SARS-CoV-2 researchers with a useful handbook covering multidisciplinary approaches on various aspects of SARS-CoV-2 research, brought together by leading laboratories across the globe. Topics covered include techniques in clinical and diagnosti...

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Bibliographic Details
Other Authors: Chu, Justin Jang Hann (Editor), Ahidjo, Bintou Ahmadou (Editor), Mok, Chee Keng (Editor)
Format: eBook
Language:English
Published: New York, NY Humana 2022, 2022
Edition:1st ed. 2022
Series:Methods in Molecular Biology
Subjects:
Online Access:
Collection: Springer eBooks 2005- - Collection details see MPG.ReNa
Table of Contents:
  • Evolution and Epidemiology of SARS-CoV-2 Virus
  • Molecular Epidemiology of SARS-CoV-2 by Sequencing
  • Advanced Genetic Methodologies in Tracking Evolution and Spread of SARS-CoV-2
  • Antigen-Based Point of Care Testing (POCT) for Diagnosing SARS-CoV-2: Assessing Performance
  • Diagnostic Method for COVID-19 Using Sugar Chain-Immobilized Nanoparticles and Saliva Specimens
  • Detection and Quantification of SARS-CoV-2 by Real-Time RT-PCR Assay
  • Immunofluorescent Antibody Techniques in the Diagnosis of SARS-CoV-2 Infection in Humans
  • Propagation and Quantification of SARS-CoV-2
  • Quantification of Infectious SARS‐CoV‐2 by the 50% Tissue Culture Infectious Dose Endpoint Dilution Assay
  • One-Step Reverse Transcription Droplet Digital PCR Protocols for SARS-CoV-2 Detection and Quantification
  • Profiling SARS-CoV-2 Infection by High-Throughput Shotgun Proteomics
  • Detection of SARS-CoV-2 by Mass Spectrometry
  • Bioinformatics-Based Approaches to Study Virus–Host InteractionsDuring SARS-CoV-2 Infection
  • Human Nasal Epithelial Cells (hNECs) Generated by Air-Liquid Interface (ALI) Culture as a Model System for Studying the Pathogenesis of SARS-CoV-2
  • Animal Models of COVID-19: Nonhuman Primates
  • Animal Models of COVID-19: Transgenic Mouse Model
  • Immunohistochemical Detection of SARS-CoV-2 Antigens, by Single and Multiple Immunohistochemistry
  • Measuring Neutralizing Antibodies to SARS-CoV-2 Using Lentiviral Spike-Pseudoviruses
  • Antiviral Strategies Against SARS-CoV-2: A Systems Biology Approach
  • Neutralization Assay for SARS-CoV-2 Infection: Plaque Reduction Neutralization Test
  • Pseudovirus-Based Assays for the Measurement of Antibody-Mediated Neutralization of SARS-CoV-2
  • Cytopathic Effect (CPE)-Based Drug Screening Assay for SARS-CoV-2
  • Strengthening Biorisk Management in Research Laboratories with Security-Sensitive Biological Agents like SARS-CoV-2
  • Biorisk Management for SARS-CoV-2 Research in a Biosafety Level-3 Core Facility
  • Methods of SARS-CoV-2 Inactivation