The Inflammasome Methods and Protocols
This volume provides inflammasome instructions and tips to study different aspects of inflammasome biology. Chapters guide readers through how to assess canonical and non-canonical inflammasome activation, in vitro immune and non-immune cells, whole blood, biochemical assays to evaluate ASC oligomer...
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| Format: | eBook |
| Language: | English |
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New York, NY
Humana
2022, 2022
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| Edition: | 1st ed. 2022 |
| Series: | Methods in Molecular Biology
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| Subjects: | |
| Online Access: | |
| Collection: | Springer eBooks 2005- - Collection details see MPG.ReNa |
Table of Contents:
- Assessment of ASC Oligomerization by Flow Cytometry
- In-vitro Assays to Study Inflammasome Activation in Primary Macrophages
- Measuring Non-Canonical Inflammasome Activation In Neutrophils
- Gasdermin D Cleavage Assay Following Inflammasome Activation
- Activation of the Non-Canonical Inflammasome In Mouse And Human Cells
- Measurement of Inflammasome-Induced Mitochondrial Dysfunction By Flow Cytometry
- Detection of ASC Oligomerization by Western Blotting
- Reconstitution System of NLRP3 Inflammasome in HEK293T Cells
- Intracellular Potassium Ions Measurements by Inductively Coupled Plasma Optical Emission Spectrometer (ICP-OES)
- NLRP3 Phospho-Residue Mapping by Phospho Dot-Blots
- Analysis of Activity and Expression of the NLRP3, AIM2 and NLRC4 Inflammasome in Whole Blood
- Measurement of Cytosolic Mitochondrial DNA after NLRP3 Inflammasome Activation
- Inflammasome-derived Exosomes Isolation and Imaging by Transmission Electron Microscopy
- In Vitro and In Vivo Model for Sepsis through Non-Canonical NLRP3 inflammasome Activation
- Inflammasome Activation in Gingival Epithelial Cells
- Imaging of Inflammasome Speck Formation in Living Cells