Non-Alcoholic Steatohepatitis Methods and Protocols
This volume contains a comprehensive collection of laboratory protocols used by researchers to analyze varied aspects of non-alcoholic steatohepatitis (NASH). The chapters in this book cover topics such as methods for histological diagnosis of NASH; the purpose of generating an in vivo NASH model; p...
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| Format: | eBook |
| Language: | English |
| Published: |
New York, NY
Humana
2022, 2022
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| Edition: | 1st ed. 2022 |
| Series: | Methods in Molecular Biology
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| Subjects: | |
| Online Access: | |
| Collection: | Springer eBooks 2005- - Collection details see MPG.ReNa |
Table of Contents:
- Histopathological Diagnosis of Nonalcoholic Steatohepatitis (NASH)
- Generation of a Diet-Induced Mouse Model of Nonalcoholic Fatty Liver Disease
- Hydrodynamic Injection for Developing NASH Model
- Measurement of Hepatic Lipids
- Measurement of Fatty Acid Oxidation in Mammalian Cells
- Measurement of In Vivo VLDL and Chylomicron Secretion
- Isolation and Culture of Mouse Hepatocytes and Kupffer Cells (KC)
- Mouse Bone Marrow Cell Isolation and Macrophage Differentiation
- Purification and Isolation of Hepatic Stellate Cells
- Isolation of the Stromal Vascular Fraction from Adipose Tissue and Subsequent Differentiation into White or Beige Adipocytes
- Culture of Mouse Liver Ductal Organoids
- Development of a Scalable Three-Dimensional Culture of Human Induced Pluripotent Stem Cells-Derived Liver Organoids
- Chromatin Immunoprecipitation Assay in Primary Mouse Hepatocytes and Mouse Liver
- Analysis of Liver Responsesto Non-Alcoholic Steatohepatitis by mRNA-Sequencing
- Single-Cell RNA-Sequencing in NASH
- Computational Pipeline for Next-Generation Sequencing (NGS) Studies in Genetics of NASH
- Metabolic Phenotyping in Mice with NASH using Indirect Calorimetry
- Analysis of Insulin Resistance in Nonalcoholic Steatohepatitis
- Assessment of Lipotoxic Endoplasmic Reticulum (ER) Stress in Nonalcoholic Steatohepatitis (NASH)
- Ex Vivo Dual-Hit Method for Inflammasome Activation in Liver
- In Vivo Analysis of Necrosis and Ferroptosis in Nonalcoholic Steatohepatitis (NASH)
- Analysis of the Sphingolipidome in NAFLD
- Bile Acid Profiling in Mouse Biofluids and Tissues
- Preparation and Characterization of a Liver Targeted, Poly(amidoamine) Based, Gene Delivery System
- Extraction of Exosomes and Exosomal miRNA from Mesenchymal Stem Cells
- Generation of Adenovirus for In Vitro and In Vivo Studies of Hepatocytes