Estrogen Receptors Methods and Protocols
This fully updated collection includes chapters on a wide array of techniques that are vital for advancing our understanding of the physiological and pathological effects of estrogen, mediated by estrogen receptor α (ERα) and estrogen receptor β (ERβ) as well as the non-genomic membrane-bound G-prot...
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| Format: | eBook |
| Language: | English |
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New York, NY
Humana
2022, 2022
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| Edition: | 2nd ed. 2022 |
| Series: | Methods in Molecular Biology
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| Subjects: | |
| Online Access: | |
| Collection: | Springer eBooks 2005- - Collection details see MPG.ReNa |
Table of Contents:
- Antibody Validation for Estrogen Receptor Beta
- Immunoblot Detection of the Phosphorylation of the Estrogen Receptor a as an Outcome of GPR30/GPER1 Activation
- Immunoprecipitation Analyses of Estrogen Receptor α Phosphorylated at Serine 216 in the Mouse Liver
- Immunofluorescent Verification of Silencing Estrogen Receptor α with siRNA in the Intact Rodent Brain
- Detection and Functional Analysis of Estrogen Receptor α Phosphorylated at Serine 216 in Mouse Neutrophils
- Quantification of Protein Expression by Proximity Ligation Assay in the Nonhuman Primate in Response to Estrogen
- In Situ Hybridization of Estrogen Receptors α and β in the Human Testis
- Extraction of RNA and Analysis of Estrogen-Responsive Genes by RT-qPCR
- PET Imaging of Estrogen Receptors Using 18F-Based Radioligands
- The Use of ERE-Luc Reporter Mice to Monitor Estrogen Receptor Transcriptional Activity in a Spatio-Temporal Dimension
- Use of Reporter Genes to Analyze Estrogen Response: The Transgenic Zebrafish Model
- Assessing Estrogenic Activity of Classical Estrogen Receptor‑Binding Compounds
- An Optimized ChIP-Seq Protocol to Determine Chromatin Binding of Estrogen Receptor Beta
- Polysome-Fractionation for Transcriptome-Wide Studies of mRNA Translation
- Anota2seq Analysis for Transcriptome-Wide Studies of mRNA Translation
- Whole-Genome Genotyping Using DNA Microarrays for Population Genetics
- Proteomics Analysis of the Estrogen Effects in the Rat Uterus Using Gel-LC and Tandem Mass Spectrometry Approaches
- Expression Profiles of Estrogen-Regulated MicroRNAs in Cancer Cells
- Molecular Cloning and Purification of the Protein Lysine Methyltransferase SMYD2 and Its Co-Crystallization with a Target Peptide from Estrogen Receptor Alpha
- Molecular Design, Synthesis, and Evaluation of SNIPER(ER) that Induces Targeted Protein Degradation of ERα
- Assessment of Metabolic Regulation by Estrogen Receptors
- RNA-Seq Experiment and Data Analysis