Immune Receptors Methods and Protocols

This volume explores immune cell receptors that are used in the detection of microbes, either by binding directly to non-self molecules or through indirectly sensing microbe-associated cellular disturbances. The chapters in this book cover methods for studying receptor-ligand interactions at both mo...

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Bibliographic Details
Other Authors: Rast, Jonathan (Editor), Buckley, Katherine (Editor)
Format: eBook
Language:English
Published: New York, NY Humana 2022, 2022
Edition:2nd ed. 2022
Series:Methods in Molecular Biology
Subjects:
Online Access:
Collection: Springer eBooks 2005- - Collection details see MPG.ReNa
Table of Contents:
  • Micropatterned Ligand Arrays to Investigate Spatial Regulation of Cellular Signaling Initiated by Clustered Fc Receptors
  • Low-Cost and Cell-Surface-Mimic Analysis of Ligand Interactions of Biotinylated Immune Receptors Using Surface Plasmon Resonance
  • Analysis of the Sub-Cellular Localization of NLRs Upon Shigella flexneri Infection Exemplified by NOD1
  • Generation of VNAR Libraries from Immunized Sharks and Selection of Target-Specific Clones
  • Identification of Glycan-Specific Variable Lymphocyte Receptors using Yeast Surface Display and Glycan Microarrays
  • Cell Aggregation Assays for Homophilic Interactions between Cell Surface Protein
  • Measuring Mutator Enzyme Activity using an E. coli-Based Colony Formation Assay
  • Lamprey Variable Lymphocyte Receptor Monoclonal Antibodies for Whole Cell Surface Antigens
  • A Role for Secreted Immune Effectors in Microbial Biofilm Formation Revealed by Simple In Vitro Assays
  • Assessment of Scavenger Receptor Cysteine Rich Domain Binding to Bacteria
  • Bioinformatics Approaches for Analyzing Multigene Families Encoding Immune Receptors
  • Production and Characterization of SIV-Specific CAR/CXCR5 T Cells
  • Engineering IgG1 Fc Domains that Activate the Complement System
  • An Imaging Flow Cytometry Protocol for Studying Immunoregulatory Receptor-Mediated Regulation of Phagocytosis
  • Single Cell Sequencing for Everybody
  • Exploring the Diversity of the B-Cell Receptor Repertoire Through High-Throughput Sequencing
  • Fetal Thymic Organ Culture (FTOC) Optimized for Gamma-Delta T Cell Studies