Fusarium wilt Methods and Protocols
This volume provides a collection of molecular protocols detailing the most common and modern techniques on fusarium wilt. Chapters guide readers through methods on initial isolation, molecular-based identification, genome characterization, generation of mutants, and characterization of interactions...
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| Format: | eBook |
| Language: | English |
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New York, NY
Humana
2022, 2022
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| Edition: | 1st ed. 2022 |
| Series: | Methods in Molecular Biology
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| Online Access: | |
| Collection: | Springer eBooks 2005- - Collection details see MPG.ReNa |
Table of Contents:
- 1. Pure culture and DNA sequence-based identification of Fusarium from symptomatic plants and diverse substrates
- Isolation of high-molecular weight (HMW) DNA from Fusarium oxysporum for long-read sequencing
- Karyotyping of Fusarium oxysporum by pulsed field gel electrophoresis and the germ tube burst method
- RNA-seq data analysis for differential expression
- Quantification and isolation of spontaneous colony growth variants
- Generation of a T-DNA insertion mutant library of Fusarium oxysporum using Agrobacterium tumefaciens
- Targeted gene disruption via CRISPR/Cas9 ribonucleoprotein complexes in Fusarium oxysporum
- CRISPR/Cas9 RNP-mediated gene fusion to assess protein quantification and subcellular localization in Fusarium oxysporum
- In vivo monitoring of cytosolic pH using the ratiometric pH sensor pHluorin
- Multi-pronged investigation of volatile compound-mediated interactions of Fusarium oxysporum with plants, fungi and bacteria
- Isolation of mitochondria from Fusarium species
- In planta gene expression analysis and colonization of Fusarium oxysporum
- Time-lapse imaging of root pathogenesis and fungal proliferation without physically disrupting roots
- High-throughput screening assays to identify plant natural products with antifungal properties against Fusarium oxysporum
- Screening and assessment of pisatin demethylase activity (PDA).-Detection and genotyping of Fusarium oxysporum f. sp. vasinfectum race 4 (VCG0114), the Fusarium wilt pathogen of cotton