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210901 ||| eng |
| 020 |
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|a 9781071615973
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| 100 |
1 |
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|a Borggrefe, Tilman
|e [editor]
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| 245 |
0 |
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|a Enhancers and Promoters
|h Elektronische Ressource
|b Methods and Protocols
|c edited by Tilman Borggrefe, Benedetto Daniele Giaimo
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| 250 |
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|a 1st ed. 2021
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| 260 |
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|a New York, NY
|b Humana
|c 2021, 2021
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| 300 |
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|a XI, 376 p. 69 illus., 57 illus. in color
|b online resource
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| 505 |
0 |
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|a A New Toolbox to Analyze Enhancer-Promoter Functions -- Global Run-on Sequencing (GRO-Seq) -- Illuminating Enhancer Transcription at Nucleotide Resolution with Native Elongating Transcript Sequencing (NET-Seq) -- Low Quantity single strand CAGE (LQ-ssCAGE) Maps Regulatory Enhancers and Promoters -- Analyses of Promoter, Enhancer and Nucleosome Organisation in Mammalian Cells by MNase-Seq -- Measuring Chromatin Accessibility: ATAC-Seq -- High-resolution ChIP-MNase Mapping of Nucleosome Positions at Selected Genomic Loci And Alleles -- Sequential Chromatin Immunoprecipitation to Identify Heterotypic Nucleosomes -- Low Input Targeted Chromatin Capture (low-T2C) -- Proximity Ligation-assisted ChIP-Seq (PLAC-Seq) -- Analysis of Enhancer-promoter Interactions using CAGE and RADICL-Seq technologies -- Using Open Chromatin Enrichment and Network Hi-C (OCEAN-C) to identify open chromatin interactions -- Assessment of 3D Interactions Between Promoters and Distal Regulatory Elements with Promoter Capture Hi-C (PCHi-C) -- Native Chromatin Proteomics (N-ChroP) to Characterize Histone Post-Translational Modification (PTM) Combinatorics At Distinct Genomic Regions -- Using ChIP-SICAP to Identify Proteins That Co-Localize in Chromatin -- Genome-wide profiling of protein-DNA Interactions with Chromatin Endogenous Cleavage And High-Throughput Sequencing (ChEC-Seq) -- Transcriptional Activation Of Heterochromatin by Recruitment of dCas9 Activators -- Deletion of Regulatory Elements with All-in-One CRISPR-Cas9 Vectors -- Simultaneous Tagmentation-based detection of ChIP/ATAC Signal with Bisulfite Sequencing -- Low Input Whole-Genome Bisulfite Sequencing
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| 653 |
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|a Medical Genetics
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| 653 |
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|a Genetics and Genomics
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| 653 |
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|a Genetics
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| 653 |
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|a Medical genetics
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| 700 |
1 |
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|a Giaimo, Benedetto Daniele
|e [editor]
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| 041 |
0 |
7 |
|a eng
|2 ISO 639-2
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| 989 |
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|b Springer
|a Springer eBooks 2005-
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| 490 |
0 |
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|a Methods in Molecular Biology
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| 028 |
5 |
0 |
|a 10.1007/978-1-0716-1597-3
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| 856 |
4 |
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|u https://doi.org/10.1007/978-1-0716-1597-3?nosfx=y
|x Verlag
|3 Volltext
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| 082 |
0 |
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|a 576.5
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| 520 |
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|a This volume contains cutting-edge techniques to study the function of enhancers and promoters in depth. Chapters are divided into six sections and describe enhancer-promoter transcripts, nucleosome occupancy, DNA accessibility, chromatin interactions, protein-DNA interactions, functional analyses, and DNA methylation assays. Written in the Methods in Molecular Biology series format, chapters include comprehensive introductions, lists of the necessary materials and reagents, step-by-step laboratory protocols, and useful suggestions for troubleshooting. Authoritative and cutting-edge, Enhancers and Promoters: Methods and Protocols is a useful guide for future experiments. Chapters 4 and 11 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com
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