Chloroplast Biotechnology Methods and Protocols

This second edition volume expands on the previous edition with updated chapters in model systems and new crops. The book contains protocols for plastid engineering in leaves, tissue culture cells, and the shoot apex of plants, as well as for marker excision from the plastid genome and engineering R...

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Bibliographic Details
Other Authors: Maliga, Pal (Editor)
Format: eBook
Language:English
Published: New York, NY Springer US 2021, 2021
Edition:2nd ed. 2021
Series:Methods in Molecular Biology
Subjects:
Online Access:
Collection: Springer eBooks 2005- - Collection details see MPG.ReNa
Table of Contents:
  • The Plastid Genomes of Flowering Plants: Essential Principles
  • Plastid Gene Transcription: An Update on Promoters and RNA Polymerases
  • Engineering Chloroplasts for High-Level Constitutive or Inducible Transgene Expression
  • Marker-Free Transplastomic Plants by Excision of Plastid Marker Genes Using Directly Repeated DNA Sequences
  • Fluorescent Labeling and Confocal Microcopy of Plastids and Stromules
  • Transformation of the Plastid Genome in Tobacco: The Model System for Chloroplast Genome Engineering
  • Nicotiana tabacum: An Update on PEG-Mediated Plastid Transformation
  • Transformation of the Plastid Genome in Tobacco Suspension Cell Cultures
  • Plastid Marker Gene Excision in the Tobacco Shoot Apex by Agrobacterium-Delivered Cre Recombinases
  • Rubisco Engineering by Plastid Transformation and Protocols for Assessing Expression
  • Plastid Transformation in Tomato: A Vegetable Crop and Model Species
  • Stable Plastid Transformation of Petunia for Studies in Basic Science
  • Plastid Transformation in Potato: An Important Source of Nutrition and Industrial Materials
  • Plastid Transformation of Poplar: A Model for Perennial Trees
  • Plastid Transformation in Lettuce (Lactuca sativa) by Biolistic DNA Delivery
  • Plastid Transformation in Sugar Beet: An Important Industrial Crop
  • A Simple Technology for Generating Marker-Free Chloroplast Transformants of the Green Alga Chlamydomonas reinhardtii
  • Regulated Chloroplast Gene Expression in Chlamydomonas
  • Plastid Transformation in Physcomitrella patens: An Update
  • Plastid Transformation of Sporelings from the Liverwort Marchantia polymorpha L..-Rapid and Modular DNA Assembly for Transformation of Marchantia Chloroplasts