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210608 ||| eng |
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|a 9781071612668
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| 100 |
1 |
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|a Weissig, Volkmar
|e [editor]
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| 245 |
0 |
0 |
|a Mitochondrial Medicine
|h Elektronische Ressource
|b Volume 2: Assessing Mitochondria
|c edited by Volkmar Weissig, Marvin Edeas
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| 250 |
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|a 2nd ed. 2021
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| 260 |
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|a New York, NY
|b Springer US
|c 2021, 2021
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| 300 |
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|a XVI, 459 p. 112 illus., 75 illus. in color
|b online resource
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| 505 |
0 |
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|a High-Throughput Image Analysis of Lipid-Droplet-Bound Mitochondria -- Cell Energy Budget Platform for Multi-Parametric Assessment of Cell and Tissue Metabolism -- Fluorescence-based Assay for Measuring OMA1 Activity -- Studying Mitochondrial Network Formation by in vivo and in vitro Reconstitution Assay -- Extraction of Functional Mitochondria Based on the Membrane Stiffness -- A Protocol for Untargeted Metabolomic Analysis: From Sample Preparation to Data Processing -- A Method for Analysis of Nitrotyrosine-Containing Proteins by Immunoblotting Coupled With Mass Spectrometry -- In vivo Visualization And Quantification of Mitochondrial Morphology in C. elegans -- Assessing Impact of Platinum Complexes on Mitochondrial Functions -- In-silico Modeling of the Mitochondrial Pumping Complexes with Markov State Models -- Monitoring the Mitochondrial Presequence Import Pathway in Living Mammalian Cells with a NewMolecular Biosensor
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| 505 |
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|a Mitochondrial Dysfunction in Mitochondrial Medicine: Current Limitations, Pitfalls, and Tomorrow -- Preparation of ‘Functional’ Mitochondria – A Challenging Business -- Isolation and Quality Control of Functional Mitochondria -- Purification of Functional Platelet Mitochondria Using a Discontinuous Percoll Gradient -- Mechanical Permeabilization as a New Method for Assessment of Mitochondrial Function in Insect Tissues -- Analysis of Mitochondrial Retrograde Signaling In Yeast Model Systems -- Native Gel Electrophoresis and Immunoblotting to Analyze Electron Transport Chain Complexes -- Measuring Mitochondrial Hydrogen Peroxide Levels and Glutathione Redox Equilibrium in Drosophila Neuron Subtypes Using Redox-Sensitive Fluorophores and 3D Imaging -- Assessment of Mitochondrial Cell Metabolism By Respiratory Chain Electron Flow Assays -- Whole-Cell and Mitochondrial dNTP Pool Quantification from Cells and Tissues --
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| 505 |
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|a Single Particle-Tracking Method in Fluorescence Microscopy to Monitor Bioenergetic Responses Of Individual Mitochondria -- Investigation of Mitochondrial ADP-ribosylation via Immunofluorescence -- Assessment of Mitochondrial Ca2+ Uptake -- Assessment of Mitochondrial Membrane Potential and NADH Redox State in Acute Brain Slices -- Evaluation of Mitochondria Content and Function in Live Cells by Multi-color Flow Cytometric Analysis -- Analysis of Mitochondrial Dysfunction during Cell Death -- Modified Blue-Native Gel Approach for Analysis of Respiratory Super-Complexes -- Patch-Clamp Recording of the Activity of Ion Channels in the Inner Mitochondrial Membrane -- Assessment of Mitochondrial Protein Glutathionylation as Signaling for CO Pathway -- 3D Optical Cryo-Imaging Method: A Novel Approach To Quantify Renal Mitochondrial Bioenergetics Dysfunction -- Simultaneous Quantification of Mitochondrial ATP and ROS Production Using ATP Energy Clamp Methodology --
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| 653 |
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|a Medicine / Research
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| 653 |
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|a Biology / Research
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| 653 |
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|a Biomedical Research
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| 700 |
1 |
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|a Edeas, Marvin
|e [editor]
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| 041 |
0 |
7 |
|a eng
|2 ISO 639-2
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| 989 |
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|b Springer
|a Springer eBooks 2005-
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| 490 |
0 |
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|a Methods in Molecular Biology
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| 028 |
5 |
0 |
|a 10.1007/978-1-0716-1266-8
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| 856 |
4 |
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|u https://doi.org/10.1007/978-1-0716-1266-8?nosfx=y
|x Verlag
|3 Volltext
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| 082 |
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|a 610.72
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| 520 |
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|a This second edition offers 88 chapters divided among three volumes providing the most comprehensive source of know-how in the wide-ranging field of Mitochondrial Medicine. Volume II guides readers through chapters on mitochondrial dysfunction, functional’ mitochondria, mitochondrial retrograde, mitochondrial dNTP pool quantification, mitochondrial ADP-ribosylation, blue-native gel approach, 3D optical cryo-imaging method, mitochondrial ATP and ROS production, protocol for untargeted metabolomic analysis, and methods for analysis of nitrotyrosine-containing proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Mitochondrial Medicine, Second Edition, Volume 2: Assessing Mitochondria aims to be a comprehensive source of know-how in the wide-ranging field of Mitochondrial Medicine
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