T-Helper Cells Methods and Protocols
The aim of this volume is to provide a comprehensive description of methods and protocols useful for the further study of T-helper cells. Chapters guide readers through T-helper cell recovery, molecular study, signal transduction pathways, T-cell manipulation and, last but not least, “omic” approach...
| Other Authors: | , , |
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| Format: | eBook |
| Language: | English |
| Published: |
New York, NY
Humana
2021, 2021
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| Edition: | 1st ed. 2021 |
| Series: | Methods in Molecular Biology
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| Subjects: | |
| Online Access: | |
| Collection: | Springer eBooks 2005- - Collection details see MPG.ReNa |
Table of Contents:
- Human and Murine T helper Cell Recovery from Organs and Tissues
- Identification and Purification of Human Memory T Helper Cells from Peripheral Blood
- Transcription Factor Evaluation by Flow Cytometry
- Mass Cytometry Analysis of T Helper Cells
- Murine T Helper Cell Differentiation and Plasticity
- Identification of Follicular T Cell Subsets in Murine and Human Tissues
- Analysis of T Cells In Inflamed Non-Lymphoid Tissues
- TCR Vβ Evaluation by Flow Cytometry
- Evaluation of Cytokine Production at the Single Cell Level by Flow Cytometry Upon Polyclonal Stimulation
- Evaluation of Secreted Cytokines by Multiplex Bead Based Assay (X MAP Technology, Luminex)
- Quantifying Antigen-specific T Cells by Assessing their Antigen-Induced Proliferation
- Flow Cytometric Characterization of Human Antigen-Reactive T helper Cells
- Identification of Human Antigen-Specific CD4+ cells with Peptide-MHC Multimer Technologies
- Human Tcell Cloning by Limiting Dilution
- Human Germinal Center Reaction: Assessing T Follicular Helper-B cell Interactions In Vitro
- Study of Signal Transduction Pathways by Phospho-Protein Evaluation
- The Study of Protein-DNA Interactions in CD4+ T Cells using ChIPmentation
- mRNA Transfection of T lymphocytes by Electroporation
- Transduction of Human T Cell Subsets with Lentivirus
- Plasmid- or Ribonucleoprotein-mediated CRISPR/Cas Gene Editing in Primary Murine T Cells
- Generation of Sequencing Libraries for Building Immune Cell Methylomes
- Gene Expression Analysis of T Cells by Single Cell RNA-seq
- Quantifying Proteome and Protein Modifications in Activated T Cells by Multiplexed Isobaric Labeling Mass Spectrometry
- Metabolomics, Lipidomics and Immunometabolism
- Experimental Mouse Models of Asthma and Analysis of CD4+ T Cells
- Murine T Cell Transfer Colitis as a Model for Inflammatory Bowel Disease
- ExperimentalAutoimmune Encephalomyelitis