Yeast Protocols
This fourth edition provides all new methods on yeast that have not been included in the past three editions. Divided in four parts, chapters guide readers through general applications for genetic manipulation in yeast cells, genome-wide studies, systematically rearrange the yeast chromosomes, and p...
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| Format: | eBook |
| Language: | English |
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New York, NY
Springer US
2021, 2021
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| Edition: | 4th ed. 2021 |
| Series: | Methods in Molecular Biology
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| Subjects: | |
| Online Access: | |
| Collection: | Springer eBooks 2005- - Collection details see MPG.ReNa |
Table of Contents:
- Use of Yeast Plasmids - Transformation and Inheritance Assays
- Construction of Tight Conditional Mutants Using the Improved Auxin-inducible Degron (iAID) Method in the Budding Yeast Saccharomyces cerevisiae
- CRISPR Nickase-Mediated Base Editing in Yeast
- Genomic Promoter Shuffling by Using Recyclable Cassettes
- Study Essential Gene Functions by Plasmid Shuffling
- Scarless Genomic Protein Labeling in Saccharomyces cerevisiae
- High-Copy Yeast Library Construction and High-Copy Rescue Genetic Screen in Saccharomyces cerevisiae
- Genome-Wide Imaging-Based Phenomic Screening Using Yeast (Saccharomyces cerevisiae) Strain Collections
- Applications of Oxford Nanopore Sequencing in Schizosaccharomyces pombe
- Measuring Genome-Wide Nascent Nucleosome Assembly Using ReIN-Map
- Targeted Integration of Complex Genetic Elements at Multi-Copy Loci by Golden Gate Assembly
- Chromosomal Rearrangements of Synthetic Yeast by SCRaMbLE
- Use YeastFab to Construct Genetic Parts and Multicomponent Pathways for Metabolic Engineering
- A Versatile Protocol to Generate Translocations in Yeast Genomes using CRISPR/Cas9
- YeastNucleoplasmic Extracts and an Application to Visualize Chromatin Assembly on Single Molecules of DNA
- Assays for Autophagy III: Observing Dynamic Protein Trafficking
- Isolation of Aged Yeast Cells Using Biotin-Streptavidin Affinity Purification
- High-Throughput Rapid Yeast Chronological Lifespan Assay
- Long-Term Imaging and Dynamic Analysis of Cytoophidia in Yeast
- Monitoring 5’-end Resection at Site-specific Double-strand Breaks by Southern Blot Analysis.