Opioid Receptors Methods and Protocols

This second edition presents an up-to-date chapters describing the most relevant and novel techniques employed to study the opioid receptors. Chapters detail transcriptional and post-transcriptional analysis, cellular detection of opioid receptors, analysis of signaling events modulated by opioid re...

Full description

Bibliographic Details
Other Authors: Spampinato, Santi M. (Editor)
Format: eBook
Language:English
Published: New York, NY Humana 2021, 2021
Edition:2nd ed. 2021
Series:Methods in Molecular Biology
Subjects:
Online Access:
Collection: Springer eBooks 2005- - Collection details see MPG.ReNa
Table of Contents:
  • Evaluating Opioid Mediated Adenylyl Cyclase Inhibition in Live Cells using a BRET-based Assay
  • Patch Clamp Analysis of Opioid-Induced Kir3 Currents in Mouse Peripheral Sensory Neurons Following Nerve Injury
  • Opioid Modulation of Neuronal Iron and Potential Contributions to NeuroHIV
  • An In Vitro Assay to Study the Role of Opioids in Modulating Immune Cell Adhesion
  • Assessing Cell Viability Effects of Opioids in Primary Cortical Cells from Rat
  • The Quest for More Effective Analgesics with Reduced Abuse Liability and Fewer Adverse Effects: Promises, Pitfalls and Future Perspectives of Biased Agonists at Opioid Receptors
  • Mechanical Nociception in Mice and Rats: Measurement with Automated von Frey Equipment
  • Evaluation of Murine Macrophage Cytokine Production after In Vivo Morphine Treatment
  • Measurement of Macrophage Toll like Receptor 4 Expression After Morphine Treatment
  • Conditioned Place Preference (CPP) in Rats: From Conditioning to Reinstatement Test
  • Analysis of Opioid-SeekingBehavior through the Intravenous Self-administration Reinstatement Model in Rats
  • Induction of a High Alcohol Consumption in Rats and Mice: Role of Opioid Receptors in Rats and Mice
  • Early Life Stress as a Probe to Study the Opioid System in Developing Rodents
  • Evaluation of m
  • opioid System Functionality in Mouse Pups: Ultrasonic Vocalizations as an Index Of Infant Attachment
  • Overview of Genetic Analysis of Human Opioid Receptors
  • Renilla luciferase Reporter Assay to Study 3’UTR-driven post-Transcriptional Regulation of OPRM1
  • Fluorescence Colocalization Analysis of Cellular Distribution of MOR-1
  • Monitoring Opioid Receptors Interaction in Living Cells by Bioluminescence Resonance Energy Transfer (BRET)
  • Bioluminescence Resonance Energy Transfer (BRET) to Detect the Interactions Between Kappa Opioid Receptor and Non-visual Arrestins
  • Functionalization and Bioconjugation of Nanoruby for Long-term, Ultrasensitive Imaging of Μ-Opioid Receptors.
  • Immunohistochemical Analysis of Opioid Receptors in Peripheral Tissues
  • Real-Time Quantitative Reverse Transcription PCR for Detection of Opioid Receptors in Immune Cells
  • Quantitative Analysis of MOR-1 Internalization in Spinal Cord of Morphine-tolerant Mice
  • GTPgS-autoradiography for Studies of Opioid Receptor Functionality