Opioid Receptors Methods and Protocols
This second edition presents an up-to-date chapters describing the most relevant and novel techniques employed to study the opioid receptors. Chapters detail transcriptional and post-transcriptional analysis, cellular detection of opioid receptors, analysis of signaling events modulated by opioid re...
Other Authors: | |
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Format: | eBook |
Language: | English |
Published: |
New York, NY
Humana
2021, 2021
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Edition: | 2nd ed. 2021 |
Series: | Methods in Molecular Biology
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Subjects: | |
Online Access: | |
Collection: | Springer eBooks 2005- - Collection details see MPG.ReNa |
Table of Contents:
- Evaluating Opioid Mediated Adenylyl Cyclase Inhibition in Live Cells using a BRET-based Assay
- Patch Clamp Analysis of Opioid-Induced Kir3 Currents in Mouse Peripheral Sensory Neurons Following Nerve Injury
- Opioid Modulation of Neuronal Iron and Potential Contributions to NeuroHIV
- An In Vitro Assay to Study the Role of Opioids in Modulating Immune Cell Adhesion
- Assessing Cell Viability Effects of Opioids in Primary Cortical Cells from Rat
- The Quest for More Effective Analgesics with Reduced Abuse Liability and Fewer Adverse Effects: Promises, Pitfalls and Future Perspectives of Biased Agonists at Opioid Receptors
- Mechanical Nociception in Mice and Rats: Measurement with Automated von Frey Equipment
- Evaluation of Murine Macrophage Cytokine Production after In Vivo Morphine Treatment
- Measurement of Macrophage Toll like Receptor 4 Expression After Morphine Treatment
- Conditioned Place Preference (CPP) in Rats: From Conditioning to Reinstatement Test
- Analysis of Opioid-SeekingBehavior through the Intravenous Self-administration Reinstatement Model in Rats
- Induction of a High Alcohol Consumption in Rats and Mice: Role of Opioid Receptors in Rats and Mice
- Early Life Stress as a Probe to Study the Opioid System in Developing Rodents
- Evaluation of m
- opioid System Functionality in Mouse Pups: Ultrasonic Vocalizations as an Index Of Infant Attachment
- Overview of Genetic Analysis of Human Opioid Receptors
- Renilla luciferase Reporter Assay to Study 3’UTR-driven post-Transcriptional Regulation of OPRM1
- Fluorescence Colocalization Analysis of Cellular Distribution of MOR-1
- Monitoring Opioid Receptors Interaction in Living Cells by Bioluminescence Resonance Energy Transfer (BRET)
- Bioluminescence Resonance Energy Transfer (BRET) to Detect the Interactions Between Kappa Opioid Receptor and Non-visual Arrestins
- Functionalization and Bioconjugation of Nanoruby for Long-term, Ultrasensitive Imaging of Μ-Opioid Receptors.
- Immunohistochemical Analysis of Opioid Receptors in Peripheral Tissues
- Real-Time Quantitative Reverse Transcription PCR for Detection of Opioid Receptors in Immune Cells
- Quantitative Analysis of MOR-1 Internalization in Spinal Cord of Morphine-tolerant Mice
- GTPgS-autoradiography for Studies of Opioid Receptor Functionality