DNA Modifications Methods and Protocols
This book provides an overview of methods and experimental protocols that are currently used to analyze the presence and abundance of non-canonical DNA nucleotides in different biological systems. Focusing particularly on the newly discovered and less studied DNA modifications that are enzymatically...
| Other Authors: | , |
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| Format: | eBook |
| Language: | English |
| Published: |
New York, NY
Humana
2021, 2021
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| Edition: | 1st ed. 2021 |
| Series: | Methods in Molecular Biology
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| Subjects: | |
| Online Access: | |
| Collection: | Springer eBooks 2005- - Collection details see MPG.ReNa |
Table of Contents:
- Analysis of 5-Carboxylcytosine Distribution Using DNA Immunoprecipitation
- High Resolution Analysis of5-Hydroxymethylcytosine by TET-Assisted Bisulfite Sequencing
- Oxidative Bisulfite Sequencing: An Experimental and Computational Protocol
- Bisulfite-Free Sequencing of 5-Hydroxymethylcytosine with APOBEC-Coupled Epigenetic Sequencing (ACE-Seq)
- Single-Nucleotide-Resolution Sequencing of N6-Methyldeoxyadenosine
- Design and Application of DNA Modification-Specific Transcription-Activator-Like Effectors
- Notes on Functional Modules in the Assembly of CRISPR/Cas9-Mediated Epigenetic Modifiers
- Analyzing DNA-Immunoprecipitation Sequencing Data
- Bioinformatics Analysis of DNA Methylation through Bisulfite Sequencing Data
- Unified Analysis of Multiple ChIP-Seq Datasets
- Integrated Analysis of DNA Methylation, Hydroxymethylation, and Gene Expression Data Using ME-Class2
- Modified Forms of Cytosine in Eukaryotes: DNA (De)Methylation and Beyond
- Evidence for Non-Cytosine Epigenetic DNA Modifications in Multicellular Eukaryotes: An Overview
- Mass Spectrometry-Based Analysis of DNA Modifications: Potential Applications in Basic Research and Clinic
- Mapping DNA Methylation in Mammals: The State of the Art
- Detection of Modified Bases in Bacteriophage Genomic DNA
- Liquid Chromatography–Mass Spectrometry Analysis of Cytosine Modifications
- Detection of DNA Methylation in Genomic DNA by UHPLC-MS/MS
- Quantification of DNA Modifications Using Two-Dimensional Ultra-Performance Liquid Chromatography Tandem Mass-Spectrometry (2D-UPLC–MS/MS)
- MS Analysis of DNA Modifications in Urinary/Body Fluids
- Preparation of Internal Standards for 2D-UPLC–MS/MS Quantification of Non-Canonical DNA Bases
- Detection of TET-Oxidized 5-Methylcytosine Bases by Capillary Gel Electrophoresis
- Immunochemical Detection of Modified Cytosine Species in Mammalian PreimplantationEmbryos
- Immunochemical Detection of Modified Cytosine Species in Lampbrush Chromatin
- Detection of Low-Abundance DNA Modifications Using Signal Amplification-Based Immunocytochemistry
- Immunohistochemical Detection of Modified Cytosine Bases in Rodent Brain
- Immunohistochemical Detection of 5-Hydroxymethylcytosine and 5-Carboxylcytosine in Sections of Zebrafish Embryos
- Immunochemical Detection of Modified Species of Cytosine in Plant Tissues
- Antigen Retrieval for Immunostaining of Modified Cytosine Species
- Computational Analysis of DNA Modifications in Confocal Images
- Three-Dimensional Confocal Analysis of Chromosome Positioning Coupled with Immunofluorescence in Mouse Sperm Nuclei
- Analysis of 5-Hydroxymethyluracil Levels Using Flow Cytometry
- Hairpin-Bisulfite PCR
- Genome-Wide Mapping of DNA Methylation 5mC by Methylated DNA Immunoprecipitation (MeDIP)–Sequencing