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200811 ||| eng |
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|a 9781592596355
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|a Hemmings, Hugh C.
|e [editor]
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|a Regulatory Protein Modification
|h Elektronische Ressource
|b Techniques and Protocols
|c edited by Hugh C. Hemmings
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|a 1st ed. 1997
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|a Totowa, NJ
|b Humana
|c 1997, 1997
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|a XIV, 446 p
|b online resource
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|a Analysis of Protein Phosphorylation in Intact Cells and Extracts -- Analysis of Protein Dephosphorylation in Intact Cells and Extracts -- Protein Phosphorylation and Dephosphorylation in Isolated Nerve Terminals (Synaptosomes) -- Protein Kinase and Phosphatase Inhibitors -- Phosphorylation State-Specific Antibodies -- Protein Tyrosine Phosphorylation -- Identification of Posttranslational Modification Sites by Site-Directed Mutagenesis -- Protein methylation in the nervous system -- Long-Chain Fatty Acylation of Proteins -- Protein ADP-Ribosylation -- Glycosylation and Glycosylphosphatidylinositol Membrane Anchors
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|a Neuroscience
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|a Neurosciences
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|a eng
|2 ISO 639-2
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|b SBA
|a Springer Book Archives -2004
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|a Neuromethods
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|a 10.1385/0896034151
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|u https://doi.org/10.1385/0896034151?nosfx=y
|x Verlag
|3 Volltext
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|a 612.8
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|a Hugh Hemmings' Regulatory Protein Modification: Techniques and Protocols offers critical reviews of major topics concerning posttranslational protein modification in neurons, as well as a comprehensive collection of state-of-the-art techniques for their analysis. Its distinguished international contributors provide detailed and specific methods for studying protein phosphorylation/dephospohorylation, methylation, long chain fatty acylation, ADP-ribosylation, glycosylation, and glycosylphosphatidylinositol anchors. Using both conventional analytical and novel molecular biological approaches, these experts discuss the techniques and reagents and cite numerous examples from the neuroscience literature in a way that allows investigators to determine the best experimental approaches for their own systems. They also offer a complete review of protein kinase and phosphatase inhibitors techniques and their applications today. Regulatory Protein Modification: Techniques and Protocols will prove an exceptionally valuable resource for both experienced and new investigators working in neuronal and nonneuronal systems
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