Antibody Engineering Protocols

Bibliographic Details
Other Authors: Paul, Sudhir (Editor)
Format: eBook
Language:English
Published: Totowa, NJ Humana 1995, 1995
Edition:1st ed. 1995
Series:Methods in Molecular Biology
Subjects:
Online Access:
Collection: Springer Book Archives -2004 - Collection details see MPG.ReNa
Table of Contents:
  • SEQHUNT
  • Molecular Modeling of Antibody-Combining Sites
  • Structure and Properties of Human Immunoglobulin Light-Chain Dimers
  • Crystallographic and Chromatographic Methods for Study of Antibody Light Chains and Other Proteins
  • Detection of Human Variable Gene Family Expression at the Single-Cell Level
  • Purification of Reduced and Alkylated Antibody Subunits
  • Murine Monoclonal Antibody Development
  • Comparative Properties of Polyclonal and Monoclonal Antibodies
  • Evaluation of Antibody Clonality
  • Affinity Immunoblotting
  • Epitope and Idiotope Mapping Using Monoclonal Antibodies
  • Anti-Idiotypic Antibodies That Mimic Opioids
  • Catalytic Antibodies
  • Preparation and Assay of Acetylcholinesterase Antibody
  • DNA Hydrolysis by Antibodies
  • Screening Strategies for Catalytic Antitransition-State Analog Antibodies
  • Expression of Chimeric Immunoglobulin Genes in Mammalian Cells
  • Single-Chain Anti-DNA Fv
  • Molecular Cloning of Antiground-State Proteolytic Antibody Fragments
  • Cloning and Bacterial Expression of an Esterolytic sFv
  • Site-Directed Mutagenesis of Antibody-Variable Regions
  • Synthetic Antibody Gene Libraries for In Vitro Affinity Maturation
  • Chaperonins in Phage Display of Antibody Fragments
  • Phage-Display Libraries of Murine and Human Antibody Fab Fragments
  • Selection of Human Immunoglobulin Light Chains from a Phage-Display Library
  • Purification of Antibody Light Chains by Metal Affinity and Protein L Chromatography
  • Rapid Purification of Recombinant Antibody Fragments for Catalysis Screening
  • Assay of Radiolabeled VIP Binding and Hydrolysis by Antibodies
  • Methods of Measuring Thyroglobulin and Peptide-Methylcoumarinamide Hydrolysis by Autoantibodies
  • Radiolabeling of Antibodies for Therapy and Diagnosis