Bacterial Artificial Chromosomes Volume 1: Library Construction, Physical Mapping, and Sequencing
The ability to transfer and maintain DNA both within and between species is an essential skill in biotechnology and medicine. In Bacterial Artificial Chromosomes, expert investigators describe not only the classic methods, but also the many novel techniques they have perfected for the transfer of la...
| Other Authors: | , |
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| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ
Humana
2004, 2004
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| Edition: | 1st ed. 2004 |
| Series: | Methods in Molecular Biology
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| Subjects: | |
| Online Access: | |
| Collection: | Springer Book Archives -2004 - Collection details see MPG.ReNa |
Table of Contents:
- BAC Library Construction
- Construction of Small Genome BAC Library for Functional and Genomic Applications
- Preparation of BAC Libraries From Bacterial Genomes by In Vitro Packaging
- Exploring Transformation-Associated Recombination Cloning for Selective Isolation of Genomic Regions
- Purification of BAC DNA
- Hybridization-Based Selection of BAC Clones
- Applications of Interspersed Repeat Sequence Polymerase Chain Reaction
- BAC Mapping Using Fluorescence In Situ Hybridization
- High-Throughput BAC Fingerprinting
- BAC End Sequencing
- Radiation Hybrid Mapping With BAC Ends
- Shotgun Library Construction for DNA Sequencing
- Rolling Circle Amplification for Sequencing Templates
- Transposon-Mediated Sequencing
- Pyrosequencing
- Use of Fimers to Eliminate Polymerase Chain Reaction and Primer-Dimer Artifacts and to Increase Yield in BAC-Sequencing Reactions
- Retrofitting BACs and PACs With LoxP Transposons to Generate Nested Deletions
- Preparing Nested-Deletion Template DNA for Field Inversion Gel Electrophoresis Analyses and Position-Specific End Sequencing With Transposon Primers
- BAC Finishing Strategies
- Using the TIGR Assembler in Shotgun Sequencing Projects
- Finishing “Working Draft” BAC Projects by Directed Sequencing With ThermoFidelase and Fimers
- Optimized Multiplex Polymerase Chain Reaction
- Assembly of DNA Sequencing Data
- Sequence Finishing
- Quality Assessment of Finished BAC Sequences