Adrenergic Receptor Protocols
In Adrenegic Receptor Protocols, Curtis Machida and a panel of expert investigators present a comprehensive collection of modern molecular methods for analyzing adrenergic receptors and corresponding second messenger systems. These proven and readily reproducible techniques utilize genetic, RNA, pro...
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| Format: | eBook |
| Language: | English |
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Totowa, NJ
Humana
2000, 2000
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| Edition: | 1st ed. 2000 |
| Series: | Methods in Molecular Biology
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| Subjects: | |
| Online Access: | |
| Collection: | Springer Book Archives -2004 - Collection details see MPG.ReNa |
Table of Contents:
- Patch-Clamp Recording Methods for Examining Adrenergic Regulation of Potassium Currents in Ocular Epithelial Cells
- Transactivator Analysis
- Southwestern Blots for Detection of a DNA Binding Protein Recognizing the ?1B-Adrenergic Receptor Gene Promoter
- DNase I Footprinting Analysis of Transcription Factors Recognizing Adrenergic Receptor Gene Promoter Sequences
- Electrophoretic Mobility Shift Assay for Detection of DNA Binding Proteins Recognizing ?-Adrenergic Receptor Gene Sequences
- Determination of mRNA Stability and Characterization of Proteins Interacting with Adrenergic Receptor mRNAs
- Microscopy Analysis
- Use of Immunohistochemistry and Confocal Microscopy in the Detection of Adrenergic Receptors
- Distribution of ?2A- and ?2C-Adrenergic Receptor Immunoreactivity in the Central Nervous System
- Quantitative Light Microscopic Autoradiography of ?2-Adrenergic Radioligand Binding Sites
- In Situ Hybridization of Adrenergic Receptor mRNA in Brain
- Use of Electron Microscopy in the Detection of Adrenergic Receptors
- Genetic Analysis
- Construction of Libraries for Isolation of Adrenergic Receptor Genes
- Isolation of Adrenergic Receptor Genes
- Analyses of Adrenergic Receptor Sequences
- Polymerase Chain Reaction Screening of Genomic Libraries for Adrenergic Receptor Genes
- Solution-Phase Library Screening for Identification of Rare Adrenergic Receptor Clones
- Genetic Polymorphisms of Adrenergic Receptors
- RNA Analysis
- Northern Blot Analyses Detecting Adrenergic Receptor mRNAs
- Ribonuclease Protection Assay for the Detection of ?1-Adrenergic Receptor RNA
- Determination of Adrenergic Receptor mRNAs by Quantitative Reverse Transcriptase-Polymerase Chain Reactions
- Nuclear Run-On Assays for Measurement of Adrenergic Receptor Transcription Rate
- Primer Extension Methods for Determination of ?1-Adrenergic Receptor mRNA Start Sites
- Expression Analysis
- Use of Eukaryotic Vectors for the Expression of Adrenergic Receptors
- Expression of ?-Adrenergic Receptors in Recombinant Baculovirus-Infected Insect Cells
- Expression of ?-Adrenergic Receptors in E. coli
- Use and Pharmacological Analysis of Established and Transfected Cell Lines Expressing Adrenergic Receptors
- Transient Transfection and Adrenergic Receptor Promoter Analysis
- Antisense RNA/DNA-Based Techniques to Probe Adrenergic Receptor Function
- Targeted Disruption of Adrenergic Receptor Genes
- Receptor and Second Messenger Analysis
- Development of Antibodies to Adrenergic Receptors
- Western Blot Detection of Adrenergic Receptors
- UV Crosslinking of Adrenergic Receptors and Ligands
- Detection of ?-Adrenergic Receptors by Radioligand Binding
- Assessing Adrenergic Receptor Conformation Using Chemically Reactive Fluorescent Probes
- Biochemical Methods for Detection and Measurement of Cyclic AMP and AdenylylCyclase Activity
- Assay of Arachidonic Acid Release Coupled to ?1- and ?2-Adrenergic Receptors