Mycoplasma Protocols
In Mycoplasma Protocols, Roger Miles and Robin Nicholas present a collection of cutting-edge methods for the detection, isolation, identification, characterization, and genetic manipulation of the pathogenic mycoplasmas. These step-by-step methods are crafted for successful reproducibility and inclu...
| Other Authors: | , |
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| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ
Humana
1998, 1998
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| Edition: | 1st ed. 1998 |
| Series: | Methods in Molecular Biology
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| Subjects: | |
| Online Access: | |
| Collection: | Springer Book Archives -2004 - Collection details see MPG.ReNa |
Table of Contents:
- Medical Significance of Mycoplasmas
- The Veterinary Significance of Mycoplasmas
- Recovery of Human Mycoplasmas
- Recovery of Mycoplasmas from Animals
- Recovery of Mycoplasmas from Birds
- Cultivation of Ureaplasmas
- Quality Control Testing of Mycoplasma Medium
- Biochemical Characteristics in Mycoplasma Identification
- Enzyme Analysis
- Determination of Substrate Utilization Rates by Mycoplasmas
- Serological Identification of Mycoplasmas by Growth and Metabolism Inhibition Tests
- Identification of Mycoplasmas by Dot Immunobinding on Membrane Filtration (MF Dot)
- Identification of Mycoplasmas by Immunofluorescence
- Diagnostic Application of Monoclonal Antibody (MAb)-Based Sandwich ELISAs
- Immunohistochemical Staining of Fixed Tissues
- Extraction of DNA from Mycoplasmas
- Characterization of Mycoplasmas by PCR and Sequence Analysis with Universal 16S rDNA Primers
- PCR and RFLP Methods for the Specific Detection and Identification of Mycoplasma mycoides subsp. mycoides SC
- Characterization of Mycoplasmas by RAPD Fingerprinting
- Classification of Isolates by DNA-DNA Hybridization
- Insertion Sequence Analysis
- Detection of Mycoplasmas in Cell Cultures by Cultural Methods
- Detection of Mycoplasmas in Cell Culture by Fluorescence Methods
- Transformation of Mycoplasmas
- Transposon Mutagenesis
- Demonstration of Extrachromosomal Elements
- Expression of Foreign Genes in Acholeplasma laidlawii
- Mycoplasma Gene Expression in Escherichia coli
- Polyacrylamide Gel-Electrophoresis Separation of Whole-Cell Proteins
- Immunological and Biochemical Characterization of Membrane Proteins
- Detection and Analysis of Mycoplasma Adhesins
- Transmission Electron Microscopy and Immunogold Staining of Mollicute Surface Antigens