Drosophila Cytogenetics Protocols

Probably no single experimental organism has contributed as much to our understanding of the structure, function, and mechanism of transmission of eukaryotic chromosomes as has the fruit fly, Drosophila melanogaster. In Drosophila Cytogenetics Protocols, leading drosophilists describe, in step-by-st...

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Bibliographic Details
Other Authors: Henderson, Daryl S. (Editor)
Format: eBook
Language:English
Published: Totowa, NJ Humana 2004, 2004
Edition:1st ed. 2004
Series:Methods in Molecular Biology
Subjects:
Online Access:
Collection: Springer Book Archives -2004 - Collection details see MPG.ReNa
Table of Contents:
  • The Chromosomes of Drosophila melanogaster
  • Spermatogenesis
  • Time-Lapse Imaging of Male Meiosis by Phase-Contrast and Fluorescence Microscopy
  • Immunocytological Analysis of Oogenesis
  • Cytological Analysis of Oogenesis
  • Polytene Chromosomes From Ovarian Nurse Cells of Drosophila melanogaster otu Mutants
  • Feulgen-DNA Cytophotometry for Estimating C Values
  • Fluorescent BrdU Labeling and Nuclear Flow Sorting of the Drosophila Ovary
  • Studying Nuclear Organization in Embryos Using Antibody Tools
  • Whole-Mount Fluorescence In Situ Hybridization to Chromosomes of Embryos
  • Orcein Staining and the Identification of Polytene Chromosomes
  • Salivary Chromosome Analysis of Aberrations
  • In Situ Hybridization to Polytene Chromosomes
  • Combined Immunostaining and FISH Analysis of Polytene Chromosomes
  • Electron Microscopy of Polytene Chromosomes
  • Analysis of Mitosis in Squash Preparations of Larval Brains
  • Analyzing Chromosome Function by High Frequency Formation of Dicentric Chromosomes In Vivo
  • Analysis of Chromosomes of the Larval CNS by FISH and BrdU Labeling
  • Immunostaining of Squash Preparations of Chromosomes of Larval Brains
  • Visualizing Mitosis in Whole-Mount Larval Brains
  • Immunostaining of Whole-Mount Imaginal Discs
  • Wing Somatic Mutation and Recombination Test
  • Analysis of Histoblasts
  • Visualizing Apoptosis
  • RNAi in Cultured Drosophila Cells