Bacterial Toxins Methods and Protocols
The interest of investigators across a broad spectrum of scientific dis- plines has been steadily stimulated by the field of bacterial toxin research, an area that makes use of a large variety of biological, chemical, physicochemical, and medically oriented approaches. Researchers studying bacterial...
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| Format: | eBook |
| Language: | English |
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Totowa, NJ
Humana
2000, 2000
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| Edition: | 1st ed. 2000 |
| Series: | Methods in Molecular Biology
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| Subjects: | |
| Online Access: | |
| Collection: | Springer Book Archives -2004 - Collection details see MPG.ReNa |
Table of Contents:
- Bacterial Protein Toxins
- Purification of Clostridium botulinum Type A Neurotoxin
- Shiga Toxins
- Isolation and Detection of Microcystins and Nodularins, Cyanobacterial Peptide Hepatotoxins
- Genetic Construction, Expression, and Characterization of Diphtheria Toxin-Based Growth Factor Fusion Proteins
- Structure-Function Analysis of Cysteine-Engineered Entomopathogenic Toxins
- Use of Fourier-Transformed Infrared Spectroscopy for Secondary Structure Determination of Staphylococcal Pore-Forming Toxins
- The Use of Fluorescence Resonance Energy Transfer to Detect Conformational Changes in Protein Toxins
- Site-Directed Spin Labeling of Proteins
- Characterization of Molecular Properties of Pore-Forming Toxins with Planar Lipid Bilayers
- Determination of Affinity and Kinetic Rate Constants Using Surface Plasmon Resonance
- ADP-Ribosylation of ?-Gi Proteins by Pertussis Toxin
- Phage Libraries for Generation of Anti-Botulinum scFv Antibodies
- T-Cell Cytotoxicity Assays for Studying the Functional Interaction Between the Superantigen Staphylococcal Enterotoxin A and T-Cell Receptors
- In Vitro Physiological Studies on Clostridial Neurotoxins
- The Biology of Endotoxin
- Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry of Lipopolysaccharides
- Applications of Combined Capillary Electrophoresis-Electrospray Mass Spectrometry in the Characterization of Short-Chain Lipopolysaccharides
- Deacylation of Lipopolysaccharides and Isolation of Oligosaccharide Phosphates
- Electrophysiological Measurements on Reconstituted Outer Membranes