MHC Protocols

Many of the genes found in the human major histocompatibility complex (MHC:HLA region) are subject to extreme polymorphism. HLA polymorphism has been used extensively in solid organ and bone marrow transplantation to match donors and recipients. Many MHC genes also have significant disease associati...

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Bibliographic Details
Other Authors: Powis, Stephen H. (Editor), Vaughan, Robert W. (Editor)
Format: eBook
Language:English
Published: Totowa, NJ Humana 2003, 2003
Edition:1st ed. 2003
Series:Methods in Molecular Biology
Subjects:
Online Access:
Collection: Springer Book Archives -2004 - Collection details see MPG.ReNa
Table of Contents:
  • Databases
  • HLA Informatics
  • Accessing HLA Sequencing Data Through the 6ace Database
  • Polymorphism in Classical and Nonclassical Hla Genes
  • HLA Typing by Restriction Fragment Length Polymorphism Analysis
  • PCR-Restriction Fragment Length Polymorphism Typing of Class I and II Alleles
  • PCR-Sequence-Specific Oligonucleotide Probe Typing for HLA-A, -B, and -DR
  • HLA-DPA1 and -DPB1 Typing Using the PCR and Nonradioactive Sequence-Specific Oligonucleotide Probes
  • PCR-Sequence-Specific Primer Typing of HLA Class I and Class II Alleles
  • HLA Typing With Reference Strand-Mediated Conformation Analysis
  • Sequencing Protocols for Detection of HLA Class I Polymorphism
  • HLA-E and HLA-G Typing
  • Typing Alleles of HLA-DM
  • Polymorphism in Non-Hla Mhc Genes
  • Typing Alleles of TAP1 and TAP2
  • Determining Alleles of the C2 Gene by Southern Blotting
  • Complement C4 Protein and DNA Typing Methods
  • Typing of Tumor Necrosis Factor Alleles
  • Molecular Typing of the MHC Class I Chain-Related Gene Locus
  • Microsatellites
  • HLA Microsatellite Analysis