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200811 ||| eng |
| 020 |
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|a 9781592597499
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| 100 |
1 |
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|a Titheradge, M. A.
|e [editor]
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| 245 |
0 |
0 |
|a Nitric Oxide Protocols
|h Elektronische Ressource
|c edited by M. A Titheradge
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| 250 |
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|a 1st ed. 1998
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| 260 |
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|a Totowa, NJ
|b Humana
|c 1998, 1998
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| 300 |
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|a XI, 324 p. 40 illus
|b online resource
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| 505 |
0 |
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|a The Determination of Nitrotyrosine Residues in Proteins -- Measurement of DNA Damage Using the Comet Assay -- Methods for the Study of NO-Induced Apoptosis in Cultured Cells
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| 505 |
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|a Measurement of eNOS and iNOS mRNA Expression Using Reverse Transcription Polymerase Chain Reaction -- Measurement of NOS mRNA by Northern Blotting and the Ribonuclease-Protection Assay -- Detection of NOS Isoforms by Western-Blot Analysis -- Immunohistochemical Localization of NOS Isoforms -- A Practical Protocol for the Demonstration of NOS Using In-Situ Hybridization -- Use of NO Donors in Biological Systems -- Making and Working with Peroxynitrite -- The Use of NO Gas in Biological Systems -- A Microtiter-Plate Assay of Human NOS Isoforms -- Use of Arginine Analogs as Inhibitors of Nitric Oxide Synthase in Rat-Aortic Rings -- Measurement of Biopterin and the Use of Inhibitors of Biopterin Biosynthesis -- A Sensitive Assay for the Enzymatic Activity of GTP Cyclohydrolase I -- Simultaneous Measurement of Mitochondrial Function and NO -- Release of NO from Donor Compounds: A Mathematical Model for Calculation of NO Concentrations in the Presence of Oxygen --
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| 505 |
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|a Enzymology of Nitric Oxide Synthases -- Purification of the Inducible Nitric Oxide Synthase -- Purification of the Constitutive Nitric Oxide Synthase -- Cloning and Expression of Human Inducible Nitric Oxide Synthase -- Cloning and Expression of Human eNOS and nNOS Using the Baculovirus-Insect Cell System -- Assay of NOS Activity by the Measurement of Conversion of Oxyhemoglobin to Methemoglobin by NO -- Measurement of NOS Activity by Conversion of Radiolabeled Arginine to Citrulline Using Ion-Exchange Separation -- Radiochemical Measurement of NOS Activity by Conversion of [14C]L-Arginine to Citrulline Using HPLC Separation -- The Enzymatic Measurement of Nitrate and Nitrite -- Determination of NOS Activity Using Cyclic-GMP Formation -- Determination of NO with a Clark-Type Electrode -- The Measurement of NO in Biological Systems Using Chemiluminescence -- Measurement of NO Using Electron Paramagnetic Resonance --
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| 653 |
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|a Biochemistry
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| 041 |
0 |
7 |
|a eng
|2 ISO 639-2
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| 989 |
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|b SBA
|a Springer Book Archives -2004
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| 490 |
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|a Methods in Molecular Biology
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| 028 |
5 |
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|a 10.1385/1592597491
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| 856 |
4 |
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|u https://doi.org/10.1385/1592597491?nosfx=y
|x Verlag
|3 Volltext
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| 082 |
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|a 572
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