Superantigen Protocols

Superantigens, a class of proteins produced by bacteria and microbes, cause diseases ranging from food poisoning to toxic shock, but can also be used to understand these pathologies and devise therapeutic agents to combat them. In Superantigen Protocols, leading researchers in the biological, chemic...

Full description

Bibliographic Details
Other Authors: Krakauer, Teresa (Editor)
Format: eBook
Language:English
Published: Totowa, NJ Humana 2003, 2003
Edition:1st ed. 2003
Series:Methods in Molecular Biology
Subjects:
Online Access:
Collection: Springer Book Archives -2004 - Collection details see MPG.ReNa
Table of Contents:
  • Superantigens
  • Expression, Purification, and Detection of Novel Streptococcal Superantigens
  • Flow Cytometric Detection of MMTV Superantigens
  • Spectrophotometric Methods for the Determination of Superantigen Structure and Stability
  • Binding Kinetics of Superantigen with TCR and MHC Class II
  • Directed Evolution of T-Cell Receptors for Binding Superantigens
  • Analysis of Superantigen Binding to Soluble T-Cell Receptors
  • Role of Accessory Molecules in the Superantigen-Induced Activation of Peripheral Blood T Cells
  • T-Lymphocyte Activation Induced by Staphylococcal Enterotoxin Superantigens
  • Measurement of Proinflammatory Cytokines and T-Cell Proliferative Response in Superantigen-Activated Human Peripheral Blood Mononuclear Cells
  • RNase Protection Assay for the Study of the Differential Effects of Therapeutic Agents in Suppressing Staphylococcal Enterotoxin B-Induced Cytokines in Human Peripheral Blood Mononuclear Cells
  • Staining Protocol for Superantigen-Induced Cytokine Production Studied at the Single-Cell Level
  • Assessment of Specific T-Cell Activation by Superantigens
  • Superantigen-Induced Changes in Epithelial Ion Transport and Barrier Function
  • Pyrogenic, Lethal, and Emetic Properties of Superantigens in Rabbits and Primates