Aqueous Two-Phase Systems Methods and Protocols
In Aqueous Two-Phase Systems: Methods and Protocols, Rajni Hatti-Kaul and her expert coauthors combine theory, methodology, and applications in a practical collection of easily reproducible protocols for bioseparations in aqueous two-phase systems (ATPS). The protocols range from established methods...
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| Format: | eBook |
| Language: | English |
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Totowa, NJ
Humana
2000, 2000
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| Edition: | 1st ed. 2000 |
| Series: | Methods in Biotechnology
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| Online Access: | |
| Collection: | Springer Book Archives -2004 - Collection details see MPG.ReNa |
Table of Contents:
- Preparation and Characterization of Aqueous Two-Phase Systems
- Aqueous Two-Phase Systems
- The Phase Diagram
- Preparation of Aqueous Two-Phase Systems
- Measurement of Some Physical Properties of Aqueous Two-Phase Systems
- Partitioning Techniques
- Single-Step Partitioning in Aqueous Two-Phase Systems
- Partition by Countercurrent Distribution (CCD)
- Liquid-Liquid Partition Chromatography (LLPC)
- Partitioning of Soluble Molecules and Particulates
- Metal Ion Separations in Aqueous Biphasic Systems and with ABEC™ Resins
- Partition of Amino Acids and Peptides in Aqueous Two-Phase Systems
- Predicting Partition Coefficients of Small Solutes Based on Hydrophobicity
- Eukaryotic Cell Partition
- Concentration and Purification of Viruses
- Isolation of Plant Plasma Membranes and Production of Inside-Out Vesicles
- Isolation of Inside-Out Thylakoid Vesicles
- Two-Phase Partitioning as a Method for Isolation of Tight Plasma Membrane Vesicles from Saccharomyces cerevisiae and from Chlamydomonas reinhardtii
- Purification of Cyanobacterial Thylakoid, Plasma, and Outer Membranes by Two-Phase Partitioning
- Purification of Plasma Membranes by Affinity Partitioning
- Studying the Influence of Salts on Partitioning of Proteins
- Partitioning of Chemically Modified Proteins
- Detection and Analysis of Interactions by Two-Phase Partition
- Cryopartitioning in Two-Phase Systems Containing Organic Solvents
- Isolation and Purification of Proteins by Two-Phase Extraction
- Optimization of Extractions in Aqueous Two-Phase Systems
- Two-Phase Extraction of Proteins from Cell Debris
- Aqueous Two-Phase Extraction of Proteins from Animal Tissue
- Chaotropic Aqueous Two-Phase Systems
- Temperature-Induced Phase Partitioning for Protein Purification
- Extraction of Amphiphilic ProteinsUsing Detergent-Based Aqueous Two-Phase Systems
- Use of Charged PEG and Dextran Derivatives for Biomolecule Partitioning
- Affinity Partitioning Using Poly(ethylene glycol) with Covalently Coupled Hydrophobic Groups
- Dye-Ligand Affinity Partitioning of Proteins
- Metal Affinity Protein Partitioning
- Recovery of Proteins and Phase Components
- Combination of Extraction with Adsorption for Protein Purification
- Integration of Extraction with Affinity Precipitation
- Affinity Partitioning Using Magnetic Two-Phase Systems
- 36 Large-Scale Extraction of Proteins
- Bioconversions in Two-Phase Systems
- Extractive Bioconversion in Aqueous Two-Phase Systems
- Enzyme Reaction in Polymer-Polymer Organic Solvent Two-Phase Systems