Tissue Engineering Methods and Protocols

Jeffrey Morgan and Martin Yarmush bring together in Tissue Engineering the first major collection of cutting-edge methods developed and used by leading researchers in the tissue engineering field. These hands-on experts describe easily reproducible protocols for the production and evaluation of poly...

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Bibliographic Details
Other Authors: Morgan, Jeffrey R. (Editor), Yarmush, Martin L. (Editor)
Format: eBook
Language:English
Published: Totowa, NJ Humana 1999, 1999
Edition:1st ed. 1999
Series:Methods in Molecular Medicine
Subjects:
Online Access:
Collection: Springer Book Archives -2004 - Collection details see MPG.ReNa
Table of Contents:
  • Measurement of Recovery of Function Following Whole Muscle Transfer, Myoblast Transfer, and Gene Therapy
  • Preparation of Immortalized Human Chondrocyte Cell Lines
  • Clinical Applications
  • Isolation and In Vitro Proliferation of Chondrocytes, Tenocytes, and Ligament Cells
  • Culture and Identification of Autologous Human Articular Chondrocytes for Implantation
  • Organogenesis of Skeletal Muscle in Tissue Culture
  • Hepatocyte Primary Cultures
  • Formation and Characterization of Hepatocyte Spheroids
  • Isolation and Culture of Human Endothelial Cells
  • Isolation and Culture of Microvascular Endothelial Cells
  • Initiation, Maintenance, and Quantification of Human Hematopoietic Cell Cultures
  • Methods to Isolate, Culture, and Study Osteoblasts
  • Cryopreservation of Rat Hepatocytes in a Three-Dimensional CultureConfiguration Using a Controlled-Rate Freezing Device
  • Cell Material Composites
  • Microencapsulation of Enzymes, Cells, and Genetically Engineered Microorganisms
  • Quantitative Measurement of the Biological Response of Cartilage to Mechanical Deformation
  • Measuring Receptor-Mediated Cell Adhesion Under Flow
  • In Vitro and In Vivo Quantification of Adhesion Between Leukocytes and Vascular Endothelium
  • Quantitative Measurement of Shear-Stress Effects on Endothelial Cells
  • Quantitative Modeling of Limitations Caused by Diffusion
  • Methods for Microencapsulation with HEMA-MMA
  • Micropatterning Cells in Tissue Engineering
  • Methods for the Serum-Free Culture of Keratinocytes and Transplantation of Collagen—GAG-Based Skin Substitutes
  • Use of Skin Equivalent Technology in a Wound Healing Model
  • Preparation and Transplantation of a Composite Graft of Epidermal Keratinocytes on Acellular Dermis
  • Development of a Bioartificial Liver Device
  • Methods for the Implantation of Liver Cells
  • Isolation and Long-Term Maintenance of Adult Rat Hepatocytes in Culture
  • Design and Fabrication of a Small Caliber Hybrid Arterial Bioprosthesis
  • Methods for the Immunoisolation and Transplantation of Pancreatic Cells
  • Methods for the Study of Nerve Cell Migration and Patterning
  • Measurement Techniques
  • Estimating Number and Volume of Islets Transplanted Within a Planar Immunobarrier Diffusion Chamber
  • Quantitative Measurement of Cell-Cell Adhesion Under Flow Conditions
  • Materials
  • Preparation of Collagen—Glycosaminoglycan Copolymers for Tissue Regeneration
  • Preparation and Use of Tethered Ligands as Biomaterials and Tools for Cell Biology
  • Nondestructive Evaluation of Biodegradable Porous Matrices for Tissue Engineering
  • Fabrication of Biodegradable Polymer Foams for Cell Transplantation and Tissue Engineering
  • Formation of Highly Porous Polymeric Foams with Controlled Release Capability
  • Magnetic-Induced Alignment of Collagen Fibrils in Tissue Equivalents
  • Preparation and Use of Thermosensitive Polymers
  • In Situ Photopolymerized Hydrogels for Vascular and Peritoneal Wound Healing
  • Fabrication and Implantation of Gel-Filled Nerve Guidance Channels
  • Small Animal Surgical and Histological Procedures for Characterizing the Performance of Tissue-Engineered Bone Grafts
  • Preparation and Use of Porous Poly(?-Hydroxyester) Scaffolds for Bone Tissue Engineering
  • Cells
  • Quantitative Assessment of Autocrine Cell Loops