Metastasis Research Protocols

Although ninety percent of fatal cancer cases involve the spread of a primary tumor, the formation of metastases is still a poorly understood, complex process and a significant problem in the treatment of cancer patients. In Metastasis Research Protocols, leading international investigators describe...

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Bibliographic Details
Other Authors: Brooks, Susan A. (Editor), Schumacher, Udo (Editor)
Format: eBook
Language:English
Published: Totowa, NJ Humana 2001, 2001
Edition:1st ed. 2001
Series:Methods in Molecular Medicine
Subjects:
Online Access:
Collection: Springer Book Archives -2004 - Collection details see MPG.ReNa
Table of Contents:
  • Selection/Segregation Cell Populations for in Vitro and in Vivo Assays of Metastatic Behavior
  • Cell Separations by Flow Cytometry
  • Immunomagnetic Cell Separation
  • Genetic Modification of Cell Lines to Enhance Their Metastatic Capability
  • In Vitro Assays of Metastatic Behavior
  • Cell Aggregation Assays
  • Cell Migration and the Boyden Chamber
  • Quantification of Cell Motility
  • In Vitro Invasion Assay Using Matrigel®
  • Membrane Invasion Culture System
  • Collagen Invasion Assay
  • Chick Heart Invasion Assay
  • Adhesion of Tumor Cells to Matrices and Endothelium
  • Assessment of Angiogenic Factors The Chick Chorioallantoic Membrane Assay
  • Methods to Evaluate the Formation and Stabilization of Blood Vessels and Their Role in Tumor Growth and Metastasis
  • Galectin-3 Binding and Metastasis
  • Animal Models of Metastasis
  • Basic Principles for the Study of Metastasis Using Animal Models
  • The Chick Embryo Metastasis Model
  • GeneTransfection for Metastasis Research Using Animal Models
  • Theoretical Considerations in Using Animal Models of Metastasis and Brief Methodology for In Vivo Colorectal Cancer Models in SCID and Nude Mice
  • Xenograft Models in Immunodeficient Animals
  • Xenograft Models in Immunodeficient Animals
  • Syngeneic Murine Metastasis Models
  • Transgenic Animal Models
  • Clinically Accurate Orthotopic Mouse Models of Cancer
  • Dissection of Tumor and Host Cells from Metastasized Organs for Testing Gene Expression Directly Ex Vivo
  • Green Fluorescent Protein for Metastasis Research