Interleukin Protocols

Interleukins, a family of proteins that regulate immunity and inflammation throughout the body, are also involved in autoimmune disease (rheumatoid arthritis, multiple sclerosis, and asthma); are implicated in such other conditions as cancer, myocardial infarction, and depression; and have value in...

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Bibliographic Details
Other Authors: O’Neill, Luke A. J. (Editor), Bowie, Andrew (Editor)
Format: eBook
Language:English
Published: Totowa, NJ Humana 2001, 2001
Edition:1st ed. 2001
Series:Methods in Molecular Medicine
Subjects:
Online Access:
Collection: Springer Book Archives -2004 - Collection details see MPG.ReNa
Table of Contents:
  • Whole Blood Assays and the Influence of Circadian Rhythmicity on Human Cytokine Measurement
  • Illustration of Methodologies Using Individual Interleukins
  • Assaying Interleukin-8
  • Measurement of Interleukin-2 by Bioassay
  • Assaying Interleukin-15
  • Assaying Interleukins In Particular Pathologies
  • Assaying Interleukin-6 in Breast Cancer
  • Cytokines, Stress, and Depressive Illness
  • Measurement of Interleukins in Cutaneous Disorders
  • Interleukins in Graves? Disease
  • Evaluation of Cytokines in Migraine Patients by ELISA
  • Assaying Interleukins in Plasma in the Course of Acute Myocardial Infarction
  • Assaying Interleukins in Particular Biological Fluids
  • Measurement of Cytokines in Peritoneal Fluids
  • Measurement of Cytokines in Seminal Plasma
  • Measurement of Cytokines in Induced Sputum
  • Cytokines in Blister Fluids
  • Measurement of Cytokinesin Synovial Fluid
  • Measurement of Cytokines in Cerebrospinal Fluid
  • Detection of Cytokines in Tears
  • General Methods for Assaying Interleukin Protein and mRNA
  • ELISAs and Interleukin Research
  • Elispot Technique for Assaying Interleukins
  • Measurement of Interleukins by ELISPOT Assay with Particular Application to Dual-Color Analysis (Stardust Assay)
  • Assaying Interleukins Intracellularly by FACS
  • Basic RT-PCR for Measurement of Cytokine Expression
  • Quantitative PCR for Measurement of Cytokine Expression
  • Single Cell RT-PCR for the Detection of Cytokine mRNA
  • Quantitation of Cytokine mRNA by Flash-Type Bioluminescence
  • Measurement of Interleukin mRNA by Northern Blotting Using a Nonradioactive Detection Method
  • Quantification of Mouse IL-6 and TNF- ? mRNA Using Xplore Assays
  • Measurement of Cytokine and Chemokine mRNA Using Nonisotopic Multiprobe RNase Protection Assay
  • Isolation of Total and Bioactive Interleukins by Immunoaffinity-Receptor Affinity Chromatography
  • Detection of Cytokines by Immunohistochemistry
  • Newer Techniques: Interleukin Signal Transduction,
  • Measurement of Phosphoinositide 3-Kinase Activation by Interleukins
  • Analysis of Interleukin-2 Signaling Using Affinity Precipitations and Polyacrylamide Gel Electrophoresis
  • Applications for Green Fluorescent Protein in Cell Signaling
  • Use of Pichia pastoris for Production of Recombinant Cytokines
  • 35Analysis of Single Nucleotide Polymorphisms in the Interleukin-1B Gene Using 5? Nuclease Assays
  • Measurement of Cytokine and Chemokine Gene Expression Patterns Using cDNA Array