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| 008 |
191115 ||| eng |
| 020 |
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|a 9781592592142
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| 100 |
1 |
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|a Jaroszeski, Mark J.
|e [editor]
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| 245 |
0 |
0 |
|a Flow Cytometry Protocols
|h Elektronische Ressource
|c edited by Mark J. Jaroszeski, Richard Heller
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| 250 |
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|a 1st ed. 1998
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| 260 |
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|a Totowa, NJ
|b Humana
|c 1998, 1998
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| 300 |
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|a X, 274 p. 56 illus
|b online resource
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| 505 |
0 |
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|a Basics of Flow Cytometry -- Detection of Terminal Transferase in Leukemia -- The Use of Flow Cytometry to Detect Intracellular Cytokine Production in Individual Cells -- Detection of Intracellular/lntranuclear Antigens -- Detection of Intracellular/lntranuclear Antigens in Tumor Cells -- Detection of Cyclins in Individual Cells by Flow and Laser Scanning Cytometry -- Estimation of Cell Viability by Flow Cytometry -- Flow Cytometric Detection of Fluorescent Redistributional Dyes for Measurement of Cell Transmembrane Potential -- Flow Cytometric Analysis of Macrophage Oxidative Metabolism Using DCFH -- Measurement of Environmental Particulate Uptake by Lung Cells Using Flow Cytometry -- Estimation of Drug Resistance by Flow Cytometry -- Flow Cytometric Monitoring of Cellular Pharmacokinetics -- Quantification of Xenoprotein Electroinsertion in Mammalian Cells -- Flow Cytometry Quantification of Electropermeabilization -- Flow Cytometric Detection and Quantitation of Cell-Cell Electrofusion Products -- Cell Cycle Analysis of Asynchronous Populations -- Simultaneous DNA/RNA Analysis of Solid and Hematoreticular Malignancies -- Solid Tumor DNA Ploidy Analysis -- FISHing for Cytokines -- Analysis of Apoptosis by Flow Cytometry -- Chromosome Sorting and Analysis by FACS -- Simultaneous Five-Six Color Multiparameter Analysis
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| 653 |
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|a Cell Biology
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| 653 |
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|a Cytology
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| 700 |
1 |
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|a Heller, Richard
|e [editor]
|
| 041 |
0 |
7 |
|a eng
|2 ISO 639-2
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| 989 |
|
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|b SBA
|a Springer Book Archives -2004
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| 490 |
0 |
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|a Methods in Molecular Biology
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| 028 |
5 |
0 |
|a 10.1385/0896033546
|
| 856 |
4 |
0 |
|u https://doi.org/10.1385/0896033546?nosfx=y
|x Verlag
|3 Volltext
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| 082 |
0 |
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|a 571.6
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| 520 |
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|a In Flow Cytometry Protocols, leading researchers and experimenters update the complex and technically sophisticated field of flow cytometry with detailed accounts of their many new, improved, and well-tested protocols and applications. Among the cutting-edge methods presented here are techniques for the detection of terminal transferase ion leukemia, intracellular cytokines, intracellular/intranuclear antigens in leukemia and tumors, and cyclins. Protocols for performing functional studies include methods for assessing viability, transmembrane potential, oxidative metabolism, environmental particulate uptake, drug resistance, and cellular pharmacokinetics. The methods for examining DNA address cell cycle analysis, DNA/RNA analysis of solid malignancies, solid-tumor DNA ploidy analysis, apoptosis, and a method that combines FISH with flow cytometry. Advanced methods for chromosome sorting and 5-6 color analysis are also detailed. Flow Cytometry Protocols contains methods that take advantage of the recently developed instrumentation and the vast array of novel probes that have led to advances in the field of flow cytometry, as well as to significantly improved standard methods. This eminently practical new book will play a key role in bringing researchers up-to-date in this unique and productive technology
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