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191115 ||| eng |
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|a 9781592595280
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| 100 |
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|a Kendall, David A.
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| 245 |
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|a Signal Transduction Protocols
|h Elektronische Ressource
|c by David A. Kendall, Stephen J. Hill
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| 250 |
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|a 1st ed. 1995
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| 260 |
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|a Totowa, NJ
|b Humana
|c 1995, 1995
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| 300 |
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|a XI, 307 p. 21 illus
|b online resource
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| 505 |
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|a The Problems and Pitfalls of Radioligand Binding -- Solubilization and Purification of a Functional Ionotropic Excitatory Amino Acid Receptor -- Autoradiography of ?1-and ?2-Adrenoceptors -- In Situ Hybridization -- Measurement of the GTPase Activity of Signal-Transducing G-Proteins in Neuronal Membranes -- Measurement of Adenylyl Cyclase Activity in Cell Membranes -- The Measurement of Cyclic AMP Levels in Biological Preparations -- Mass Measurements of Cyclic AMP Formation by Radioimmunoassay, Enzyme Immunoassay, and Scintillation Proximity Assay -- Measurement of Cyclic GMP Formation -- Cyclic AMP-Dependent Protein Kinase Activity Ratio Assay -- Purification and Activation of Cyclic GMP-Dependent Protein Kinase -- The Analysis and Assay of Cyclic Nucleotide Phosphodiesterase Isoenzyme Activity -- Separation of Labeled Inositol Phosphate Isomers by High-Pressure Liquid Chromatography (HPLC) -- Mass Measurement of Key Phosphoinositide Cycle Intermediates -- Measurement of Phospholipase C Activity in Brain Membranes -- Measurement of Diacylglycerol -- Measurement of Intracellular Free Calcium Ion Concentration in Cell Populations Using Fura-2 -- Measurement of Ca2+ Fluxes in Permeabilized Cells Using 45Ca2+ and Fluo-3 -- Single-Cell Calcium Imaging -- Characterization of Calcium/Calmodulin-Stimulated Protein Kinase II -- Protein Kinase C -- The Measurement of Phospholipase D-Linked Signaling in Cells -- Phospholipase A2 Activity -- Techniques for the Measurement of Nitric Oxide
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| 653 |
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|a Cell Biology
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| 653 |
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|a Cytology
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| 700 |
1 |
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|a Hill, Stephen J.
|e [author]
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| 041 |
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7 |
|a eng
|2 ISO 639-2
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| 989 |
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|b SBA
|a Springer Book Archives -2004
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| 490 |
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|a Methods in Molecular Biology
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| 028 |
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|a 10.1385/0896032981
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| 856 |
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|u https://doi.org/10.1385/0896032981?nosfx=y
|x Verlag
|3 Volltext
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| 082 |
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|a 571.6
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| 520 |
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|a As our understanding of the biological sciences expands, the bou- aries between traditional disciplines tend to blur at the edges. Physio- gists and pharmacologists, for instance, now need to embrace techniques that until recently were the strict preserves of biochemists and mole- lar biologists. However, the acquisition of new technologies can be a time-consuming and frustrating business, and unless an expert is on hand to give instruction, precious hours can be spent poring over half-described Methods sections with no guarantee of eventual success. The aim of Signal Transduction Protocols has been to get experts with "hands-on" experience in particular techniques to give detailed accounts of experimental protocols in a recipe-type format, which we hope will circumvent the problems of ambiguity often encountered when reading the literature. The techniques described in Signal Transduction Protocols are those that we think will be most useful in addressing questions in the area of receptor-mediated cell signaling, with particular regard to those receptors that are part of the G-protein-linked superfamily. To keep it to a manageable size, we have omitted any reference to electrophysi- ogy and have instead concentrated on more biochemical approaches
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