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191115 ||| eng |
| 020 |
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|a 9781592595068
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| 100 |
1 |
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|a Graham, John M.
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| 245 |
0 |
0 |
|a Biomembrane Protocols
|h Elektronische Ressource
|b I. Isolation and Analysis
|c by John M. Graham, Joan A. Higgins
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| 250 |
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|a 1st ed. 1993
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| 260 |
|
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|a Totowa, NJ
|b Humana
|c 1993, 1993
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| 300 |
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|a X, 314 p
|b online resource
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| 505 |
0 |
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|a The Identification of Subcellular Fractions from Mammalian Cells -- The Isolation of Nuclei and Nuclear Membranes from Rat Liver -- Isolation of Mitochondria, Mitochondrial Membranes, Lysosomes, Peroxisomes, and Golgi Membranes from Rat Liver -- Continuous-Flow Electrophoresis -- Fractionation of a Microsomal Fraction from Rat Liver or Cultured Cells -- Isolation of Plasma Membrane Sheets and Plasma Membrane Domains from Rat Liver -- The Analysis of Receptor-Mediated Endocytosis by Centrifugation in Isoosmotic Gradients -- The Isolation of Clathrin-Coated Vesicles and Purification of Their Protein Components -- Isolation of Membranes from Tissue Culture Cells -- The Isolation of Membranes from Bacteria -- Isolation and Purification of Functionally Intact Chloroplasts from Leaf Tissue and Leaf Tissue Protoplasts -- Isolation and Purification of Functionally Intact Mitochondria from Plant Cells -- Isolation of Cholinergic-Specific Synaptosomes by Immunoadsorption -- Separation and Analysis of Phospholipids by Thin Layer Chromatography -- Analysis of Phospholipids by High Performance Liquid Chromatography -- Measurement of Cholesterol in Membranes -- Analysis of Fatty Acids by Gas Liquid Chromatography -- Chemical Assays for Proteins -- Separation and Analysis of Membrane Proteins by SDS-Polyacrylamide Gel Electrophoresis -- Analysis of Membrane Proteins by Western Blotting and Enhanced Chemiluminescence -- Two-Dimensional Polyacrylamide Gel Electrophoresis of Membrane Proteins -- Isolation of a Membrane-Bound Enzyme, 5?-Nucleotidase -- The Isolation of Membrane Proteoglycans -- Oligosaccharide Mapping and Sequence Analysis of Glycosaminoglycans -- Analysis of Sugar Sequences in Glycoproteins by Glycosidase Digestion and Gel Filtration -- The Extraction and Analysis of Glycosphingolipids
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| 653 |
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|a Cell Biology
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| 653 |
|
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|a Cytology
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| 700 |
1 |
|
|a Higgins, Joan A.
|e [author]
|
| 041 |
0 |
7 |
|a eng
|2 ISO 639-2
|
| 989 |
|
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|b SBA
|a Springer Book Archives -2004
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| 490 |
0 |
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|a Methods in Molecular Biology
|
| 028 |
5 |
0 |
|a 10.1385/0896032361
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| 856 |
4 |
0 |
|u https://doi.org/10.1385/0896032361?nosfx=y
|x Verlag
|3 Volltext
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| 082 |
0 |
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|a 571.6
|