Animal Cell Culture
For many years I performed tissue culture in large scientific insti- tions that had a great deal of infrastructure. When I set up a tissue l- oratory outside such an infrastructure, however, I found there was a shortage of easily accessible information about the basic needs, reagents, and techniques...
| Other Authors: | , |
|---|---|
| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ
Humana
1990, 1990
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| Edition: | 1st ed. 1990 |
| Series: | Methods in Molecular Biology
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| Subjects: | |
| Online Access: | |
| Collection: | Springer Book Archives -2004 - Collection details see MPG.ReNa |
Table of Contents:
- In Situ Hybridization with Radiolabeled cRNA Probes, Using Tissue Sections and Smears
- Immunogold Labeling for Electron Microscopy
- Human Chromosome Analysis and Sorting
- Flow Cytometry
- Fluorescence-Activated Cell Sorting According to Receptor Density
- Transfection and Transformation of Human Thyroid Epithelial Cells
- Expression of Foreign Genes in Cultured Insect Cells Using a Recombinant Baculovirus Vector
- Chloramphenicol Acetyltransferase as a Reporter in Mammalian Gene Transfer
- Immunizing Schedules for Hybridoma Production
- Fusion Protocol for the Production of Mouse Hybridomas
- Cloning of Hybridomas
- Enzyme-Linked Immunosorbent Assay for Screening of Antibodies in Hybridoma Supernatants
- Cryopreservation of Hybridomas
- Isotype Determination of Monoclonal Antibodies by Immunodiffusion
- Isoelectric Focusing and Immunofixation of Monoclonal Antibodies
- Large-Scale Production and Storage of Monoclonal Antibodies and Hybridomas
- Purification of Murine Monoclonal Antibodies
- Rat x Rat Hybridomas
- Monoclonal Antibodies Against Glycosphingolipids (GSLs)-Gangliosides
- Basic Cell Culture
- Establishment, Maintenance, and Cloning of Human Primary Cell Strains
- Aging of Cultured Human Skin Fibroblasts
- Separation and Maintenance of Primary T and B Lymphocytes
- Establishment of Lymphoblastoid Cell Lines
- Scale-Up of Suspension and Anchorage-Dependent Animal Cells
- Mycoplasma Detection
- Short-Term Chorionic Villi and Amniotic Fluid Cultures
- Keratinocyte Culture
- Establishment of Mouse Epithelial Lung Cell Strains and Cell Lines
- Culture of Human Brain Tumors on an Extracellular Matrix Derived from Bovine Corneal Endothelial Cells and Cultured Human Glioma Cells
- Embryonic Rodent Brain Cells in Culture
- Human Thyroid Epithelial Cells
- Preparation of Isolated Rat Liver Hepatocytes
- Primary Cultures of Rat Hepatocytes
- Preparation of Isolated Periportal or Perivenous Hepatocytes from Rat Liver
- Primary Kidney Cells
- Adipocytes
- Tissue Culture of Skeletal Muscle
- Derivation and Maintenance of Embryonic Stem Cell Cultures
- Defining Hormone and Matrix Requirements for Differentiated Epithelia
- Culturing Primitive Hemopoietic Cells
- High Proliferative Potential Colony Forming Cells
- Murine Bone Marrow-Derived Macrophages
- Long-Term B Lymphoid Cultures from Murine Bone Marrow
- CFU-C in Agar
- Human Long-Term Bone Marrow Culture
- In Vitro Clonal Culture of Human Hematopoietic Progenitor Cells
- Flow Sorting for Isolating CFU-E
- Production of Human and Murine Eosinophils In Vitro and Assay for Eosinophil Differentiation Factors
- Clonogenic Assays for Hematopoietic and Tumor Cells Using Agar-Containing Capillaries
- Cytogenetic Techniques for Human Leukemias
- Time-Lapse Cinemicroscopy
- Transmission and Scanning Electron Microscope Preparation of Whole Cultured Cells
- Double Indirect-Immunofluorescent Labeling of Cultured Cells
- Gene Mapping to Chromosomes by Hybridization in Situ