Germ Cell Protocols Volume 1: Sperm and Oocyte Analysis
Germ cell research has produced enormous advances in recent years and more recently has entered into an explosive phase of new discoveries with the introduction of transgenic technologies and nuclear cloning. In Germ Cell Protocols, expert laboratory investigators describe in step-by-step detail a s...
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| Format: | eBook |
| Language: | English |
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Totowa, NJ
Humana
2004, 2004
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| Edition: | 1st ed. 2004 |
| Series: | Methods in Molecular Biology
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| Online Access: | |
| Collection: | Springer Book Archives -2004 - Collection details see MPG.ReNa |
Table of Contents:
- Cryopreservation of Semen of the Salmonidae with Special Reference to Large-Scale Fertilization
- Fertilization and Sperm Chemotaxis in Ascidians
- Assays for Vertebrate Sperm Chemotaxis
- Stallion Spermatozoa Viability
- Visualization of Sperm Accessory Structures in the Mammalian Spermatids, Spermatozoa, and Zygotes by Immunofluorescence, Confocal, and Immunoelectron Microscopy
- Localization of Zona Pellucida Receptors on Live Sperm by Fluorophore-Conjugated Solubilized Zona Pellucida Proteins
- Detection of Human Papillomavirus DNA in Sperm Using Polymerase Chain Reaction
- Spermatogonial Stem Cells
- Molecular and Cellular Approaches for Establishment of an In Vitro Spermatogenesis System
- Acrosin Activity Assay for the Evaluation of Mammalian Sperm Acrosome Reaction
- Isolation of Sea Urchin Sperm Plasma Membranes
- Detection of Centrosome Structure in Fertilized and Artificially Activated Sea Urchin Eggs Using Immunofluorescence Microscopy and Isolation of Centrosomes Followed by Structural Characterization with Field Emission Scanning Electron Microscopy
- Microscopic Techniques for Studying Sperm-Oocyte Interaction During Fertilization and Early Embryonic Development
- Immunocytochemical Staining of the Metaphase II Spindle in Mammalian Oocytes
- Spindle Imaging in Living Mammalian Oocytes Using the Liquid-Crystal Polarized Light Microscope and Its Practical Use
- In Vitro Maturation and Fertilization of Pig Oocytes
- In Vitro Maturation and In Vitro Fertilization of Human Oocytes
- In Vitro Maturation and Fertilization of Canine Oocytes
- Detection and Measurement of Membrane-Bound Protein Tyrosine Kinases in the Zebrafish Egg
- Single-Cell Method for Estimation of Protein Tyrosine Kinases in the Zebrafish Egg
- Activity of MAPK/p90rsk During Fertilization inMice, Rats, and Pigs