Adipose Tissue Protocols
Adipose tissue is now recognized as a widely dispersed secretory organ that exhibits autocrine, paracrine, and endocrine properties, and plays a significant role in obesity, the most common health problem in industrialized countries. In Adipose Tissue Protocols, Gérard Ailhaud and a team of laborato...
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| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ
Humana
2001, 2001
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| Edition: | 1st ed. 2001 |
| Series: | Methods in Molecular Biology
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| Subjects: | |
| Online Access: | |
| Collection: | Springer Book Archives -2004 - Collection details see MPG.ReNa |
Table of Contents:
- Choosing an Adipose Tissue Depot for Sampling
- Morphologic Techniques for the Study of Brown Adipose Tissue and White Adipose Tissue
- Confocal Microscopy of Adipocytes
- Cellularity Measurements
- Subcellular Fractionation of Adipocytes and 3T3-L1 Cells
- Quantification of Lipid-Related mRNAs by Reverse Transcription-Competitive Polymerase Chain Reaction in Human White Adipose Tissue Biopsies
- Glycogen Synthase Activity in Adipose Tissue
- Assays of Lipolytic Enzymes
- Assays of Lipogenic Enzymes
- Assays of Adrenergic Receptors
- Transfection of Adipocytes and Preparation of Nuclear Extracts
- Assay of Membrane Transport of Long-Chain Fatty Acids by Adipocytes
- Assays of Glucose Entry, Glucose Transporter Amount, and Translocation
- Methods to Study Phosphorylation and Activation of the Hormone-Sensitive Adipocyte Phosphodiesterase Type 3B in Rat Adipocytes
- Measurements of Glucose Conversion to its Metabolites
- Metabolite and Ion Fluxes and Ion Channels in Brown and White Adipocytes
- Culture of Adipose Tissue and Isolated Adipocytes
- Cultures of Adipose Precursor Cells from Brown Adipose Tissue and of Clonal Brown-Adipocyte-Like Cell Lines
- Cultures of Adipose Precursor Cells and Cells of Clonal Lines from Animal White Adipose Tissue
- Cultures of Human Adipose Precursor Cells
- Measurements of Peptide and Nonpeptide Secretory Products from Adipocytes
- Assessment of White Adipose Tissue Metabolism by Measurement of Arteriovenous Differences
- Measurement of Adipose Tissue Blood Flow
- Respiratory and Thermogenic Capacities of Cells and Mitochondria from Brown and White Adipose Tissue
- Measurements of White Adipose Tissue Metabolism by Microdialysis Technique
- Immunoassay Measurements of Leptin