PCR Cloning Protocols
In the post-genomic era, PCR has become the method of choice not only for cloning existing genes, but also for generating a wide array of novel genes by mutagenesis and/or recombination within the genes of interest. PCR Cloning Protocols, Second Edition, updates and expands Bruce White's best-s...
| Other Authors: | , |
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| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ
Humana
2002, 2002
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| Edition: | 2nd ed. 2002 |
| Series: | Methods in Molecular Biology
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| Subjects: | |
| Online Access: | |
| Collection: | Springer Book Archives -2004 - Collection details see MPG.ReNa |
Table of Contents:
- Performing and Optimizing PCR
- Polymerase Chain Reaction
- Computer Programs for PCR Primer Design and Analysis
- Single-Step PCR Optimization Using Touchdown and Stepdown PCR Programming
- XL PCR Amplification of Long Targets from Genomic DNA
- Coupled One-Step Reverse Transcription and Polymerase Chain Reaction Procedure for Cloning Large cDNA Fragments
- Long Distance Reverse-Transcription PCR
- Increasing PCR Sensitivity for Amplification from Paraffin-Embedded Tissues
- GC-Rich Template Amplification by Inverse PCR
- PCR Procedure for the Isolation of Trinucleotide Repeats
- Methylation-Specific PCR
- Direct Cloning of Full-Length Cell Differentially Expressed Genes by Multiple Rounds of Subtractive Hybridization Based on Long-Distance PCR and Magnetic Beads
- Cloning PCR Products
- Cloning PCR Products
- Using T4 DNA Polymerase to Generate Clonable PCR Products
- Enzyme-Free Cloning of PCR Products and Fusion Protein Expression
- Directional Restriction Site-Free Insertion of PCR Products into Vectors
- Autosticky PCR
- A Rapid and Simple Procedure for Direct Cloning of PCR Products into Baculoviruses
- Mutagenesis and Recombination
- PCR Approaches to DNA Mutagenesis and Recombination
- In-Frame Cloning of Synthetic Genes Using PCR Inserts
- Megaprimer PCR
- PCR-Mediated Recombination
- PCR Method for Generating Multiple Mutations at Adjacent Sites
- A Fast Polymerase Chain Reaction-Mediated Strategy for Introducing Repeat Expansions into CAG-Repeat Containing Genes
- PCR Screening in Signature-Tagged Mutagenesis of Essential Genes
- Staggered Extension Process (StEP) In Vitro Recombination
- Random Mutagenesis by Whole-Plasmid PCR Amplification
- Cloning Unknown Neighboring DNA
- PCR-Based Strategies to Clone Unknown DNA Regions from Known Foreign Integrants.-Long Distance Vectorette PCR (LDV PCR)
- Nonspecific, Nested Suppression PCR Method for Isolation of Unknown Flanking DNA (“Cold-Start Method”)
- Inverse PCR
- 31 Inverse PCR
- Gene Cloning and Expression Profiling by Rapid Amplification of Gene Inserts with Universal Vector Primers
- The Isolation of DNA Sequences Flanking Tn5 Transposon Insertions by Inverse PCR
- Rapid Amplification of Genomic DNA Sequences Tagged by Insertional Mutagenesis
- Isolation of Large-Terminal Sequences of BAC Inserts Based on Double-Restriction-Enzyme Digestion Followed by Anchored PCR
- A #x201C;Step Down#x201D; PCR-Based Technique for Walking Into and the Subsequent Direct-Sequence Analysis of Flanking Genomic DNA
- Library Construction and Screening
- Use of PCR in Library Screening
- Cloning of Homologous Genes by Gene-Capture PCR
- Rapid and Nonradioactive Screening of Recombinant Libraries by PCR
- Rapid cDNA Cloning by PCR Screening (RC-PCR)
- Generation and PCR Screening of Bacteriophage ? Sublibraries Enriched for Rare Clones (the “Sublibrary Method ”)
- PCR-Based Screening for Bacterial Artificial Chromosome Libraries
- A 384-Well Microtiter-Plate-Based Template Preparation and Sequencing Method
- A Microtiter-Plate-Based High Throughput PCR Product Purification Method