Malaria Methods and Protocols
Despite considerable scientific and medical effort over the past decades, malaria remains the most important human parasitic disease. It is responsible for up to 3 million deaths and another 300-500 million new cases each year, and is becoming resistant to the current chemoprophylactic and chemother...
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| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ
Humana
2002, 2002
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| Edition: | 1st ed. 2002 |
| Series: | Methods in Molecular Medicine
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| Subjects: | |
| Online Access: | |
| Collection: | Springer Book Archives -2004 - Collection details see MPG.ReNa |
Table of Contents:
- Analysis of CSA-Binding Parasites and Antiadhesion Antibodies
- Analysis of the Adhesive Properties of Plasmodium falciparum-Infected Red Blood Cells Under Conditions of Flow
- Preparation of Adhesive Targets for Flow-Based Cytoadhesion Assays
- Triton X-114 Phase Partitioning for Antigen Characterization
- Immunoprecipitation for Antigen Localization
- Testing Interventions For Malaria Control
- Field Trials
- Assessing Risk of Infection and Severity of Disease
- Vector Incrimination and Entomological Inoculation Rates
- Epidemiological Measures of Risk of Malaria
- Laboratory Models
- Maintenance of the Plasmodium berghei Life Cycle
- Mouse Models for Pre-Erythrocytic-Stage Malaria
- Mouse Models for Erythrocytic-Stage Malaria
- Nonhuman Primate Models
- Nonhuman Primate Models
- Diagnosis and Typing
- Vector Analysis
- Genotyping of Plasmodium spp.
- Genotyping of Plasmodium falciparum
- Microsatellite Analysis in Plasmodium falciparum
- Quantitation of Liver-Stage Parasites by Automated TaqMan® Real-Time PCR
- Quantitation of Liver-Stage Parasites by Competitive RT-PCR
- Molecular Biology Techniques
- Extraction and Purification of Plasmodium Total RNA
- Extraction and Purification of Plasmodium Parasite DNA
- Southern Blotting of Parasite DNA
- SDS-PAGE and Western Blotting of Plasmodium falciparum Proteins
- Nested PCR Analysis of Plasmodium Parasites
- RFLP Analysis
- Analysis of Gene Expression by RT-PCR
- In Situ Detection of RNA in Blood- and Mosquito-Stage Malaria Parasites
- Purification of Chromosomes from Plasmodium falciparum
- Construction of Genomic Libraries from the DNA of Plasmodium Species
- Construction of a Gene Library with Mung Bean Nuclease-Treated Genomic DNA
- Construction of Plasmodium falciparum ? cDNA Libraries
- Production of Stage-Specific Plasmodium falciparum cDNA Libraries Using Subtractive Hybridization
- Construction and Screening of YAC Libraries
- Episomal Transformation of Plasmodium berghei
- Gene Targeting in Plasmodium berghei
- Immunological Techniques
- Peptide Vaccination
- DNA Vaccination
- Assessing Antigen-Specific CD8+ and CD4+ T-Cell Responses in Mice After Immunization with Recombinant Viruses
- Assessing CD4+HelperT-Lymphocyte Responses by Lymphoproliferation
- Limiting Dilution Analysis of Antigen-Specific CD4+ T-Cell Responses in Mice
- Cell Trafficking
- Assessing Antigen-Specific Proliferation and Cytokine Responses Using Flow Cytometry
- Cytokine Analysis by Intracellular Staining
- Assessment of Antigen-Specific CTL- and CD8+-Dependent IFN-? Responses in Mice
- Assessing Antigen-Specific CD8+ CTL Responses in Humans
- Human Antibody Subclass ELISA
- Systemic Nitric Oxide Production in Human Malaria
- Systemic Nitric Oxide Production in Human Malaria
- Cell Biology Techniques
- In Vitro Culture of Plasmodium Parasites
- Automated Synchronization of Plasmodium falciparum Parasites by Culture in a Temperature-Cycling Incubator
- Hepatic Portal Branch Inoculation
- Hepatocyte Perfusion, Isolation, and Culture
- Inhibition of Sporozoite Invasion
- Inhibition of Liver-Stage Development Assay
- T-Cell Mediated Inhibition of Liver-Stage Development Assay
- Antibody-Dependent Cellular Inhibition Assay
- Erythrocytic Malaria Growth or Invasion Inhibition Assays with Emphasis on Suspension Culture GIA