Asthma

As asthma increases in prevalence and severity around the world, the need for understanding its basic mechanisms and for developing better drugs and preventative measures becomes more urgent. In Asthma: Mechanisms and Protocols, K. Fan Chung and Ian Adcock bring together the first collection of labo...

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Bibliographic Details
Other Authors: Chung, K. Fan (Editor), Adcock, Ian (Editor)
Format: eBook
Language:English
Published: Totowa, NJ Humana 2000, 2000
Edition:1st ed. 2000
Series:Methods in Molecular Medicine
Subjects:
Online Access:
Collection: Springer Book Archives -2004 - Collection details see MPG.ReNa
Table of Contents:
  • Asthma: Application of Cell and Molecular Biology Techniques to Unravel Causes and Pathophysiological Mechanisms
  • Culture of Normal Human Airway Epithelial Cells and Measurement of Mucin Synthesis and Secretion
  • Brush Biopsy and Culture of Airway Epithelial Cells: ?-Adrenergic Receptor System Function
  • Isolation and Characterization of Human Airway Fibroblasts in Culture
  • Flow Cytometric Analysis of Blood Monocytes and Alveolar Macrophages
  • Expression of IL-10 and GM-CSF in Blood Monocytes and Alveolar Macrophages
  • MAP Kinase Expression in Eosinophils
  • Human Eosinophil Isolation and the Measurement of Apoptosis
  • Adhesion of T-Cells to Airway Smooth Muscle Cells
  • Proliferation of Circulating T-Cells
  • Differential Display Analysis of Inflammatory Genes in Airway Epithelium
  • Analysis of Transcription Factor Activation: NF?B as a Regulator of Inflammatory Genes in Epithelial Cells
  • Transient Transgenic Approaches for Investigating the Role of GM-CSF in Pulmonary Inflammation and Immune Diseases
  • Quantitative Analysis of RANTES mRNA in Endobronchial Biopsies Using Polymerase Chain Reaction
  • Intracellular Cytokine Staining for Analysis by Flow Cytometry
  • Localization of IL-4 and IL-5 mRNA in Bronchial Biopsies
  • Immunohistochemical Analysis of Adhesion Molecules in Airway Biopsies
  • Site-Directed Mutagenesis in the Investigation of ?-Adrenoreceptor Exosite
  • Methods for Determining ?2-Adrenoreceptor Genotype
  • Modulation of ?-Adrenoreceptor Expression in the Airways
  • Chemical Mutational Analysis of Glucocorticoid Receptor
  • Histone Acetylation and Deacetylation
  • Genome-Wide Search for Asthma Genes