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191115 ||| eng |
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|a 9781592597703
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| 100 |
1 |
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|a Luo, Z. David
|e [editor]
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| 245 |
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|a Pain Research
|h Elektronische Ressource
|b Methods and Protocols
|c edited by Z. David Luo
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| 250 |
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|a 1st ed. 2004
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| 260 |
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|a Totowa, NJ
|b Humana
|c 2004, 2004
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| 300 |
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|a XII, 304 p
|b online resource
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| 505 |
0 |
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|a Mechanistic Dissection of Pain -- Assessment of Acute Thermal Nociception in Laboratory Animals -- Tissue Injury Models of Persistent Nociception in Rats -- Segmental Spinal Nerve Ligation Model of Neuropathic Pain -- Partial Sciatic Nerve Transection -- Modeling Diabetic Sensory Neuropathy in Rats -- Sciatic Inflammatory Neuropathy in the Rat -- A Rat Pain Model of Vincristine-Induced Neuropathy -- Loop Dialysis Catheter -- Intrathecal Catheterization and Drug Delivery in the Rat -- Trigeminal Neuronal Recording in Animal Models of Orofacial Pain -- In Vivo Electrophysiology of Dorsal-Horn Neurons -- Single-Fiber Recording -- Anatomical Identification of Neurons Responsive to Nociceptive Stimuli -- Isolation and Culture of Sensory Neurons From the Dorsal-Root Ganglia of Embryonic or Adult Rats -- Primary Cultures of Neonatal Rat Spinal Cord -- Single-Cell Laser-Capture Microdissection and RNA Amplification -- Semi-Quantitative Real-Time PCR for Pain Research -- Functional Genomic Analysis in Pain Research Using Hybridization Arrays -- Generation of Transgenic Mice -- Knockout Mouse Models in Pain Research
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| 653 |
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|a Neurology
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| 653 |
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|a Neurology
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| 041 |
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7 |
|a eng
|2 ISO 639-2
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| 989 |
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|b SBA
|a Springer Book Archives -2004
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| 490 |
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|a Methods in Molecular Medicine
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| 028 |
5 |
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|a 10.1385/159259770X
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| 856 |
4 |
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|u https://doi.org/10.1385/159259770X?nosfx=y
|x Verlag
|3 Volltext
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| 082 |
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|a 616.8
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| 520 |
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|a Despite significant advances in molecular biology techniques and in our understanding of the physiology and the behavioral pharmacology of pain transduction, effective, target-specific therapeutic agents for chronic pain are still lacking. In Pain Research: Methods and Protocols, leading researchers who have first-hand experience describe, in step-by-step detail, diverse and novel techniques for dissecting the molecular mechanisms of pain transduction. These readily reproducible methods employ a variety of multidisciplinary approaches ranging from animal pain models and single neutron selection to in vitro single-cell mRNA amplification. The collection includes not only standard, cutting-edge methods, but also novel techniques only recently applied to pain research. The protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Versatile and easy-to-use, Pain Research: Methods and Protocols offers today's pain researchers in academic and pharmaceutical laboratories powerful tools to unravel the cellular and molecular complexity of pain transduction and set the stage for the next generation of pain medications
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