Stem Cells and Aging Methods and Protocols

This fully updated book collects protocols that reflect current investigations into how stem cell populations change during organismal aging. These methodologies are well-established and described in easy to follow fashion so as to be valuable for not only experts but novices in the ever-developing...

Full description

Bibliographic Details
Other Authors: Turksen, Kursad (Editor)
Format: eBook
Language:English
Published: New York, NY Humana 2019, 2019
Edition:2nd ed. 2019
Series:Methods in Molecular Biology
Subjects:
Online Access:
Collection: Springer eBooks 2005- - Collection details see MPG.ReNa
Table of Contents:
  • Generation of Retinal Pigmented Epithelial (RPE) Cells for Treatment of Age-Related Macular Degeneration (AMD)
  • Histopathological and Behavioral Assessments of Aging Effects on Stem Cell Transplants in an Experimental Traumatic Brain Injury
  • 3D Age-Specific Mortality Trajectory: A Survival Analysis Protocol
  • Isolation, Expansion, and Characterization of Wharton’s Jelly-Derived Mesenchymal Stromal Cell: Method to Identify Functional Passages for Experiments
  • CRISPR Base Editing in Induced Pluripotent Stem Cells
  • Targeted, Amplicon-Based,Next-Generation Sequencing to Detect Age-Related Clonal Hematopoiesis
  • Column Free Method for Isolation and Culture of C-Kit Positive Stem Cells from Atrial Explants
  • Histological Assessment of Cre/loxP Genetic Recombination in the Aging Subventricular Zone of Nestin-CreERT2/Rosa26YFP Mice
  • Infrared Spectroscopy and Imagıng in Stem Cells and Agıng Research
  • Use of U-STELA for Accurate Measurement of Extremely Short-Telomeres
  • Surface-Antigen-Based Identification of In Vitro Expanded Skeletal-Muscle-Derived Mesenchymal Stromal/Stem Cells Using Flow Cytometry
  • Analysis of Stem Cells and Their Activity in Human Skeletal Muscles by Immunohistochemistry
  • Methods for Detection of Autophagy in Mammalian Cells
  • Metabolomic and Proteomic Analyses of Mouse Primordial Germ Cells
  • Reprogramming of Aged Cells into Pluripotent Stem Cells by Nuclear Transfer
  • Assessing Muscle Stem Cell Clonal Complexity during Aging
  • Simultaneous Isolation of Stem and Niche Cells of Skeletal Muscle: Applicability for Aging Studies
  • Isolation and Culture of Individual Myofibers and Their Adjacent Muscle Stem Cells from Aged and Adult Skeletal Muscle
  • Methods and Strategies for Procurement, Isolation, Characterization, and Assessment of Senescence of Human Mesenchymal Stem Cells from Adipose Tissue
  • Quantifying Senescence-Associated Phenotypes in Primary Multipotent Mesenchymal Stromal Cell Cultures
  • Adipogenic and Osteogenic Differentiation of In Vitro Aged Human Mesenchymal Stem Cells
  • Human Skeletal-Muscle-Derived Mesenchymal Stem/Stromal Cell Isolation and Growth Kinetics Analysis
  • Complete Assessment of Multilineage Differentiation Potential of Human Skeletal-Muscle-Derived Mesenchymal Stem/Stromal Cells
  • Human Synovium-Derived Mesenchymal Stem Cells: Ex Vivo Analysis
  • 3D-Embedded Cell Cultures to Study Tendon Biology