Mass Spectrometry of Proteins Methods and Protocols

New insights into modern medicine and systems biology are enabled by innovative protocols and advanced technologies in mass spectrometry-based proteomics. This volume details new pipelines, workflows, and ways to process data that allow for new frontiers in proteomics to be pushed forward. With appl...

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Bibliographic Details
Other Authors: Evans, Caroline A. (Editor), Wright, Phillip C. (Editor), Noirel, Josselin (Editor)
Format: eBook
Language:English
Published: New York, NY Humana 2019, 2019
Edition:1st ed. 2019
Series:Methods in Molecular Biology
Subjects:
Online Access:
Collection: Springer eBooks 2005- - Collection details see MPG.ReNa
Table of Contents:
  • Next Generation Proteomics for Clinical Biomarker Detection using SWATH-MS
  • A Combined Chemical Derivatization/Mass Spectrometric Method for the Enhanced Detection and Relative Quantification of Protein Ubiquitination
  • Assessment of Ubiquitin Chain Topology by Targeted Mass Spectrometry
  • Quantitative Phosphoproteomic using Titanium Dioxide Micro-Columns and Label Free Quantitation
  • Isotopic Labeling and Quantitative Proteomics of Acetylation on Histones and Beyond
  • Quantitative Analysis of Protein S-Acylation Site Dynamics Using Site-Specific Acyl-Biotin Exchange (ssABE)
  • Reducing Complexity? Cysteine Reduction and S-alkylation in Proteomic Workflows: Practical Considerations
  • Detection of Unknown Chemical Adduct Modifications on Proteins: From Wet to Dry Laboratory
  • Considerations for Identifying Endogenous Protein Complexes from Tissue via Immunoaffinity Purification and Quantitative Mass Spectrometry
  • Metaproteomics of Freshwater Microbial Communities
  • Peptide-to-Protein Summarization: An Important Step for Accurate Quantification In Label-Based Proteomics
  • Experimental Design in Quantitative Proteomics
  • Practical Integration of Multi-Run iTRAQ Data
  • Quantitative Proteomics Data in the Public Domain: Challenges and Opportunities
  • Computational Proteomics with Jupyter and Python
  • The Galaxy Platform for Reproducible Affinity Proteomic Mass Spectrometry Data Analysis