G Protein-Coupled Receptor Signaling Methods and Protocols

This detailed volume assembles comprehensive protocols to assist with the study of structural, molecular, cell biological, and in vivo facets of GPCRs, and to enable the development of experimental tools for screening novel GPCR drugs. Sections explore the tweaking of ligands, bioluminescence and FR...

Full description

Bibliographic Details
Other Authors: Tiberi, Mario (Editor)
Format: eBook
Language:English
Published: New York, NY Humana 2019, 2019
Edition:1st ed. 2019
Series:Methods in Molecular Biology
Subjects:
Online Access:
Collection: Springer eBooks 2005- - Collection details see MPG.ReNa
LEADER 04422nmm a2200337 u 4500
001 EB001870102
003 EBX01000000000000001033473
005 00000000000000.0
007 cr|||||||||||||||||||||
008 190802 ||| eng
020 |a 9781493991211 
100 1 |a Tiberi, Mario  |e [editor] 
245 0 0 |a G Protein-Coupled Receptor Signaling  |h Elektronische Ressource  |b Methods and Protocols  |c edited by Mario Tiberi 
250 |a 1st ed. 2019 
260 |a New York, NY  |b Humana  |c 2019, 2019 
300 |a XV, 406 p. 87 illus., 71 illus. in color  |b online resource 
505 0 |a Optical Modulation of Metabotropic Glutamate Receptor Type 5 In Vivo Using a Photoactive Drug -- Imaging of Tissue-Specific and Temporal Activation of GPCR Signaling Using DREADD Knock-In Mice -- Laser Doppler Flowmetry to Study the Regulation of Cerebral Blood Flow by G Protein-Coupled Receptors in Rodents -- Preparation of Pulmonary Artery Myocytes and Rings to Study Vasoactive GPCRs 
505 0 |a Assessment of Conformational State Transitions of Class B GPCRs Using Molecular Dynamics -- Molecular Dynamic Simulations to Probe Water Permeation Pathways of GPCRs -- Expression and Purification of a Functional E. coli 13CH3-Methionine-Labeled Thermostable Neurotensin Receptor 1 Variant for Solution NMR Studies -- A Combined Cell-Free Protein Synthesis and Fluorescence-Based Approach to Investigate GPCR Binding Properties -- Furan Cross-Linking Technology for Investigating GPCR-Ligand Interactions -- Optical Regulation of Class C GPCRs by Photoswitchable Orthogonal Remotely-Tethered Ligands -- Chemoselective Acylation of Hydrazinopeptides to Access Fluorescent Probes for Time-Resolved FRET Assay on GPCRs -- Time-Resolved FRET-Based Assays to Characterize G Protein-Coupled Receptor Hetero-Oligomer Pharmacology -- Combining Conformational Profiling of GPCRs with CRISPR/Cas9 Gene Editing Approaches --  
505 0 |a Measuring GPCR Stoichiometry Using Types -1, -2 and -3 Bioluminescence Resonance Energy Transfer-Based Assays -- Combining SRET2 and BiFC to Study GPCR Heteromerization and Protein-Protein Interactions -- Quantification and Comparison of Signals Generated by Different FRET-Based cAMP Reporters -- Measuring GPCR-Induced Activation of Protein Tyrosine Phosphatases (PTP) Using In-Gel and Colorimetric PTP Assays -- Measurement of β-Arrestin Recruitment at GPCRs Using the Tango Assay -- Probing the Interactome of Corticotropin-Releasing Factor Receptor Heteromers Using Mass Spectrometry -- Monitoring the Aggregation of GPCRs by Fluorescence Microscopy -- Combining RNAi and Immunofluorescence Approaches to Investigate Post-Endocytic Sorting of GPCRs into Multivesicular Bodies -- Super-Resolution Imaging of G Protein-Coupled Receptors Using Ground State Depletion Microscopy -- Analysis of Spatial Assembly of GPCRs Using Photoactivatable Dyes and Localization Microscopy --  
653 |a Neuroscience 
653 |a Neurosciences 
653 |a Protein Biochemistry 
653 |a Pharmacology 
653 |a Proteins  
041 0 7 |a eng  |2 ISO 639-2 
989 |b Springer  |a Springer eBooks 2005- 
490 0 |a Methods in Molecular Biology 
028 5 0 |a 10.1007/978-1-4939-9121-1 
856 4 0 |u https://doi.org/10.1007/978-1-4939-9121-1?nosfx=y  |x Verlag  |3 Volltext 
082 0 |a 572.6 
520 |a This detailed volume assembles comprehensive protocols to assist with the study of structural, molecular, cell biological, and in vivo facets of GPCRs, and to enable the development of experimental tools for screening novel GPCR drugs. Sections explore the tweaking of ligands, bioluminescence and FRET approaches, specific GPCR signaling properties, as well as visualization of subcellular compartmentalization. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, G Protein-Coupled Receptor Signaling: Methods and Protocols serves as an ideal reference for life scientists working in a variety of research fields including molecular pharmacology, cell and developmental biology, brain behavior and physiology, drug development and screening. Chapter 4 is available open access under a CC BY 4.0 license via link.springer.com