Synapse Development Methods and Protocols
This detailed volume collects protocols for experimentation into how neurons connect to produce the extraordinary functionalities of the nervous system. Contributed by experts and pioneers in their respective techniques, the book covers synapses in the brain and in culture, their constituents, their...
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| Format: | eBook |
| Language: | English |
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New York, NY
Humana
2017, 2017
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| Edition: | 1st ed. 2017 |
| Series: | Methods in Molecular Biology
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| Subjects: | |
| Online Access: | |
| Collection: | Springer eBooks 2005- - Collection details see MPG.ReNa |
Table of Contents:
- A Cell Culture System to Investigate the Presynaptic Control of Subsynaptic Membrane Differentiation at the Neuromuscular Junction
- Co-Culture Synaptogenic Assay: A New Look at Fluorescence Reporters and Technological Devices
- Synaptogenic Assays Using Neurons Cultured on Micropatterned Substrates
- Monitoring Synapses Via Trans-Synaptic GFP Complementation
- Generation of Spinal Motor Neurons from Human Pluripotent Stem Cells
- Biochemical Purification of Binding Partners of Synaptic Scaffold Proteins
- In Situ Protein Binding Assay Using Fc-Fusion Proteins
- ation Microscopy for the Investigation of Synaptic Structure and Function
- 3D dSTORM Imaging of Fixed Brain Tissue
- Photomarking Relocalization Technique for Correlated Two-Photon and Electron Microcopy Imaging of Single Stimulated Synapses
- 3D Analysis of Synaptic Ultrastructure in Organotypic Hippocampal Slice Culture by High-Pressure Freezing and ElectronTomography
- Analyzing Endosomal Docking, Fusion, Sorting, and Budding Mechanisms in Isolated Organelles
- Concurrent Imaging of Receptor Trafficking and Calcium Dynamics by Spinning Disk Confocal Microscopy
- Imaging Activity-Dependent Signaling Dynamics at the Neuronal Synapse Using FRET-Based Biosensors
- Analyzing Structural Plasticity of Dendritic Spines in Organotypic Slice Culture
- Using Fluorescent Markers to Estimate Synaptic Connectivity In Situ
- Dual Anterograde and Retrograde Viral Tracing of Reciprocal Connectivity
- Mapping Synaptic Inputs of Developing Neurons Using Calcium Imaging
- Monosynaptic Tracing in Developing Circuits Using Modified Rabies Virus