ERK Signaling Methods and Protocols
This volume provides a collection of techniques and approaches for the study of ERK signaling. It begins with a historical perspective of genetic and molecular discoveries, followed by chapters covering specific topics in a broad range of experimental systems, including in vitro assays of EGFR and E...
| Other Authors: | |
|---|---|
| Format: | eBook |
| Language: | English |
| Published: |
New York, NY
Springer New York
2017, 2017
|
| Edition: | 1st ed. 2017 |
| Series: | Methods in Molecular Biology
|
| Subjects: | |
| Online Access: | |
| Collection: | Springer eBooks 2005- - Collection details see MPG.ReNa |
Table of Contents:
- Analyzing ERK Signal Dynamics during Zebrafish Somitogenesis
- Modeling RASopathies with Genetically Modified Mouse Models
- Dissecting Cell-Fate Determination through Integrated Mathematical Modeling of the ERK/MAPK Signaling Pathway
- How Genetics has Helped Piece Together the MAPK Signaling Pathway
- In Vitro Enzyme Kinetics Analysis of EGFR
- High-Throughput Analysis of Mammalian Receptor Tyrosine Kinase Activation in Yeast Cells
- Structural Studies of ERK2 Protein Complexes
- Isolation and Characterization of Intrinsically Active (MEK-Independent) Mutants of Mpk1/Erk
- Assaying Activation and Subcellular Localization of ERK in Cells and Tissues
- Detection and Functional Analysis of SUMO-Modified MEK
- Single-Step Affinity Purification of ERK Signaling Complexes Using the Streptavidin-Binding Peptide (SBP) Tag
- High-Throughput In Vitro Identification of Direct MAPK/Erk Substrates
- Global Identification of ERK Substrates by Phosphoproteomics Based on IMAC and 2D-DIGE
- Analysis of Ras/ERK Compartmentalization by Subcellular Fractionation
- Cell-Based Assays to Study ERK Pathway/Caveolin1 Interactions
- The Nuclear Translocation of ERK
- Visualization of RAS/MAPK Signaling In Situ by the Proximity Ligation Assay (PLA)
- Measuring ERK Activity Dynamics in Single Living Cells Using FRET Biosensors
- Quantifying Tensile Force and ERK Phosphorylation on Actin Stress Fibers
- Co-Culture Activation of MAP Kinase in Drosophila S2 Cells
- Isolation of Mouse Embryonic Stem Cell Lines in the Study of ERK1/2 MAP Kinase Signaling
- 3D Organotypic Culture Model to Study Components of ERK Signaling
- Genetic Validation of Cell Proliferation via Ras-Independent Activation of the Raf/Mek/Erk Pathway
- Genome-Wide Analysis of RAS/ERK Signaling Targets
- Probing Chromatin Modifications in Response to ERK Signaling
- Analyzing pERK Activation during Planarian Regeneration
- Discovering Functional ERK Substrates Regulating Caenorhabditis elegans Germline Development
- Reconstructing ERK Signaling in the Drosophila Embryo from Fixed Images
- Using CRISPR-Cas9 to Study ERK Signaling in Drosophila