Vaccinia Virus Methods and Protocols
This detailed book provides practical information for the laboratory that can be applied to the study of vaccinia and other poxviruses while emphasizing long-standing field standards and focusing on emerging new technologies applied in the field of poxvirology. The methods and protocols have been de...
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| Format: | eBook |
| Language: | English |
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New York, NY
Humana
2019, 2019
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| Edition: | 1st ed. 2019 |
| Series: | Methods in Molecular Biology
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| Subjects: | |
| Online Access: | |
| Collection: | Springer eBooks 2005- - Collection details see MPG.ReNa |
Table of Contents:
- Working Safely with Vaccinia Virus: Laboratory Technique and Review of Published Cases of Accidental Laboratory Infections with Poxviruses
- Bioinformatics for Analysis of Poxvirus Genomes
- Simple, Rapid Preparation of Poxvirus DNA for PCR Cloning and Analysis
- Construction and isolation of recombinant vaccinia virus expressing fluorescent proteins
- Generation of Vaccinia Virus Gene Deletion Mutants Using Complementing Cell Lines
- Vaccinia genome editing using CRISPR.-RNAi-mediated depletion of poxvirus proteins
- Assessing the Structure and Function of Vaccinia Virus Gene Products by Transient Complementation
- Preliminary Screening and In vitro confirmation of orthopoxvirus antivirals
- Vaccinia virus transcriptome analysis by RNA sequencing
- Ribosome profiling of vaccinia virus-infected cells
- Quantitative PCR-based Assessment of Vaccinia Virus RNA and DNA in Infected Cells
- Click Chemistry Based Labelling of Poxvirus Genomes
- Visualizing poxvirus replication and recombination using live cell imaging
- High-Content Analyses of Vaccinia Plaque Formation
- Super-Resolution Microscopy of Vaccinia Virus Particles
- Bioluminescence imaging as a tool for poxvirus biology
- Growth and Purification of Vaccinia Virus Stocks for Intravital Imaging
- Intravital Imaging of Vaccinia Virus-Infected Mice