Hydrocarbon and Lipid Microbiology Protocols Microbial Quantitation, Community Profiling and Array Approaches
This Volume presents methods for quantifying microbial populations and characterising microbial communities by extracting and analysing biomarkers such as RNA, DNA and lipids. The chapters cover a wide range of topics, including: cell separation from oil-rich environments, enumeration of hydrocarbon...
| Other Authors: | , , |
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| Format: | eBook |
| Language: | English |
| Published: |
Berlin, Heidelberg
Springer Berlin Heidelberg
2017, 2017
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| Edition: | 1st ed. 2017 |
| Series: | Springer Protocols Handbooks
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| Subjects: | |
| Online Access: | |
| Collection: | Springer eBooks 2005- - Collection details see MPG.ReNa |
Table of Contents:
- Introduction to Microbial Quantitation, Community Profiling, and Array Approaches
- A cell extraction method for oily sediments
- Introduction to microplate MPN-enumeration of hydrocarbon degraders
- Primers for dsr genes and Most Probable Number Method for detection of sulfate-reducing bacteria in oil reservoirs
- Microbial biomass and community analysis by PLFA analysis
- Real-time PCR approaches for analysis of hydrocarbon-degrading bacterial communities
- Clone libraries of ribosomal RNA gene sequences for characterization of microbial communities
- Denaturing gradient gel electrophoresis (DGGE) for microbial community analysis
- Microbial community profiling: SSCP and T-RFLP techniques
- Microbial community analysis by single-amplicon high-throughput sequencing (metagenetics) – preparation, data handling and ecological analysis
- Multiplex fluorescent antibody microarrays and antibody graphs for microbial and biomarker detection in the environment
- Studying protistan communitiesin hydrocarbon-contaminated environments