Collagen Methods and Protocols

This detailed volume compiles state-of-the-art protocols that will serve as recipes for scientists researching collagen, an abundant protein with great importance to health and disease, as well as in applications like food, cosmetics, pharmaceuticals, cosmetic surgery, artificial skin, and glue. Beg...

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Bibliographic Details
Other Authors: Sagi, Irit (Editor), Afratis, Nikolaos A. (Editor)
Format: eBook
Language:English
Published: New York, NY Humana 2019, 2019
Edition:1st ed. 2019
Series:Methods in Molecular Biology
Subjects:
Online Access:
Collection: Springer eBooks 2005- - Collection details see MPG.ReNa
Table of Contents:
  • Cloning and Mutagenesis Strategies for Large Collagens
  • Multi-Well Fibrillation Assay as a Tool for Analyzing Crosslinking and Stabilization of Collagen
  • Production and Characterization of Chemically Cross-Linked Collagen Scaffolds
  • A Biomimetic Model for Mineralization of Type-I Collagen Fibrils
  • Investigation of Triple-Helix Collagen Hydroxylation by Solid-State NMR Spectroscopy
  • Measurement of Collagen Crosslinks from Tissue Samples by Mass Spectrometry
  • Mass Spectrometry-Based Proteomics to Define Intracellular Collagen Interactomes
  • Exploring Extracellular Matrix Degradomes by TMT-TAILS N-Terminomics
  • Sample Preparation of Extracellular Matrix of Murine Colons for Scanning Electron Microscopy
  • In Situ Detection of Degraded and Denatured Collagen via Triple Helical Hybridization: New Tool in Histopathology
  • Combination of Traction Assays and Multiphoton Imaging to Quantify Skin Biomechanics
  • Analysis of Collagen-Binding Integrins Interactions with Supramolecular Aggregates of the Extracellular Matrix
  • Assessing Collagen Deposition during Aging in Mammalian Tissue and in Caenorhabditis elegans
  • Procollagen C-Proteinase Enhancer 1 (PCPE-1) in Liver Fibrosis
  • Tumorigenic Interplay between Macrophages and Collagenous Matrix in the Tumor Microenvironment
  • FACS Analysis of Col1α Protein Levels in Primary Fibroblasts
  • Methods for the Construction of Collagen-Based Triple-Helical Peptides Designed as Matrix Metalloproteinase Inhibitors