BCL-2 Family Proteins Methods and Protocols
This volume explores numerous techniques for the genetic, molecular, biochemical, and structural examination of BCL-2 family proteins and their interactions. The chapters in this book cover topics such as the relevance of BCL-2 proteins in health and disease; evaluating cellular dependencies to spec...
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| Format: | eBook |
| Language: | English |
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New York, NY
Humana
2019, 2019
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| Edition: | 1st ed. 2019 |
| Series: | Methods in Molecular Biology
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| Subjects: | |
| Online Access: | |
| Collection: | Springer eBooks 2005- - Collection details see MPG.ReNa |
Table of Contents:
- Overview of BCL-2 Family Proteins and Therapeutic Potentials
- Database and Bioinformatic Analysis of BCL-2 Family Proteins and BH3-Only Proteins
- Application of Mito-Priming to Generate BCL-2 Addicted Cells
- BH3 Profiling: A Functional Assay to Measure Apoptotic Priming and Dependencies
- Flow Cytometry-Based Detection and Analysis of BCL-2 Family Proteins and Mitochondrial Outer Membrane Permeabilization (MOMP)
- Investigating BCL-2 Family Protein Interactions in Yeast
- Liposomal Permeabilization Assay to Study the Functional Interaction of the BCL-2 Family
- Mitochondrial Isolation, Gel Filtration, and Real-Time Monitoring of MOMP
- Photocrosslinking Approach to Investigate Protein Interactions in the BCL-2 Family
- Assessment of Dynamic BCL-2 Protein Shuttling between Outer Mitochondrial Membrane and Cytosol
- Quantification of BCL-2 Family Members by Flow Cytometry
- Methods to Probe Calcium Regulation by BCL-2 FamilyMembers
- Methods to Probe Conformational Activation and Mitochondrial Activity of Proapoptotic BAK
- Probing BAK and BAX Activation and Pore Assembly with Cytochrome c Release, Limited Proteolysis and Oxidant-Induced Linkage
- BCL-2 Protein Family Interaction Analysis by Nuclear Magnetic Resonance Spectroscopy
- Reconstitution and Characterization of BCL-2 Family Proteins in Lipid Bilayer Nanodiscs
- Cryo-Electron Microscopy to Study Bax Pores and MOMP
- CW EPR and DEER Methods to Determine BCL-2 Family Protein Structure and Interactions: Application of Site-Directed Spin Labeling to BAK Apoptotic Pores
- Rapid Imaging of BCL-2 Family Interactions in Live Cells using FLIM-FRET
- Quantification of the Interactions between BCL-2 Proteins by Fluorescence Correlation Spectroscopy
- Isolation of Synthetic Antibodies against BCL-2-Associated X Protein (BAX)